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1.B.12.1.2
Autoexporter of virulence factor G, VirG or IcsA

Accession Number:Q7BCK4
Protein Name:Outer membrane protein icsA autotransporter
Length:1102
Molecular Weight:116244.00
Species: [623]
Location1 / Topology2 / Orientation3: Cell outer membrane1 / Multi-pass membrane protein2
Substrate Virulence factor G

Cross database links:

HEGENOM: HBG683627
RefSeq: NP_085346.1    NP_858315.1   
Entrez Gene ID: 1238021    876473   
Pfam: PF03797    PF03212   
KEGG: sfl:CP0182   

Gene Ontology

GO:0009279 C:cell outer membrane
GO:0009986 C:cell surface
GO:0005576 C:extracellular region
GO:0016021 C:integral to membrane
GO:0042597 C:periplasmic space
GO:0005886 C:plasma membrane
GO:0009405 P:pathogenesis

References (17)

[1] “virG, a plasmid-coded virulence gene of Shigella flexneri: identification of the virG protein and determination of the complete coding sequence.”  Lett M.-C.et.al.   2644195
[2] “The virulence plasmid pWR100 and the repertoire of proteins secreted by the type III secretion apparatus of Shigella flexneri.”  Buchrieser C.et.al.   11115111
[3] “Complete DNA sequence and analysis of the large virulence plasmid of Shigella flexneri.”  Venkatesan M.M.et.al.   11292750
[4] “Comparison of two major forms of the Shigella virulence plasmid pINV: positive selection is a major force driving the divergence.”  Lan R.et.al.   14573649
[5] “Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157.”  Jin Q.et.al.   12384590
[6] “Unipolar localization and ATPase activity of IcsA, a Shigella flexneri protein involved in intracellular movement.”  Goldberg M.B.et.al.   8468279
[7] “Cleavage of Shigella surface protein VirG occurs at a specific site, but the secretion is not essential for intracellular spreading.”  Fukuda I.et.al.   7896693
[8] “A dual transcriptional activation system for the 230 kb plasmid genes coding for virulence-associated antigens of Shigella flexneri.”  Adler B.et.al.   2474742
[9] “Identification of icsA, a plasmid locus of Shigella flexneri that governs bacterial intra- and intercellular spread through interaction with F-actin.”  Bernardini M.L.et.al.   2542950
[10] “Phosphorylation of IcsA by cAMP-dependent protein kinase and its effect on intracellular spread of Shigella flexneri.”  d'Hauteville H.et.al.   1602963
[11] “Extracellular transport of VirG protein in Shigella.”  Suzuki T.et.al.   8537341
[12] “Neural Wiskott-Aldrich syndrome protein is implicated in the actin-based motility of Shigella flexneri.”  Suzuki T.et.al.   9582270
[13] “Activation of the CDC42 effector N-WASP by the Shigella flexneri IcsA protein promotes actin nucleation by Arp2/3 complex and bacterial actin-based motility.”  Egile C.et.al.   10491394
[14] “The unipolar Shigella surface protein IcsA is targeted directly to the bacterial old pole: IcsP cleavage of IcsA occurs over the entire bacterial surface.”  Steinhauer J.et.al.   10231492
[15] “Polar targeting of Shigella virulence factor IcsA in Enterobacteriacae and Vibrio.”  Charles M.et.al.   11481451
[16] “IcsA, a polarly localized autotransporter with an atypical signal peptide, uses the Sec apparatus for secretion, although the Sec apparatus is circumferentially distributed.”  Brandon L.D.et.al.   14507362
[17] “Escape of intracellular Shigella from autophagy.”  Ogawa M.et.al.   15576571
Structure:
3ML3     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNQIHKFFCN MTQCSQGGAG ELPTVKEKTC KLSFSPFVVG ASLLLGGPIA FATPLSGTQE 
61:	LHFSEDNYEK LLTPVDGLSP LGAGEDGMDA WYITSSNPSH ASRTKLRINS DIMISAGHGG 
121:	AGDNNDGNSC GGNGGDSITG SDLSIINQGM ILGGSGGSGA DHNGDGGEAV TGDNLFIING 
181:	EIISGGHGGD SYSDSDGGNG GDAVTGVNLP IINKGTISGG NGGNNYGEGD GGNGGDAITG 
241:	SSLSVINKGT FAGGNGGAAY GYGYDGYGGN AITGDNLSVI NNGAILGGNG GHWGDAINGS 
301:	NMTIANSGYI ISGKEDDGTQ NVAGNAIHIT GGNNSLILHE GSVITGDVQV NNSSILKIIN 
361:	NDYTGTTPTI EGDLCAGDCT TVSLSGNKFT VSGDVSFGEN SSLNLAGISS LEASGNMSFG 
421:	NNVKVEAIIN NWAQKDYKLL SADKGITGFS VSNISIINPL LTTGAIDYTK SYISDQNKLI 
481:	YGLSWNDTDG DSHGEFNLKE NAELTVSTIL ADNLSHHNIN SWDGKSLTKS GEGTLILAEK 
541:	NTYSGFTNIN AGILKMGTVE AMTRTAGVIV NKGATLNFSG MNQTVNTLLN SGTVLINNIN 
601:	APFLPDPVIV TGNMTLEKNG HVILNNSSSN VGQTYVQKGN WHGKGGILSL GAVLGNDNSK 
661:	TDRLEIAGHA SGITYVAVTN EGGSGDKTLE GVQIISTDSS DKNAFIQKGR IVAGSYDYRL 
721:	KQGTVSGLNT NKWYLTSQMD NQESKQMSNQ ESTQMSSRRA SSQLVSSLNL GEGSIHTWRP 
781:	EAGSYIANLI AMNTMFSPSL YDRHGSTIVD PTTGQLSETT MWIRTVGGHN EHNLADRQLK 
841:	TTANRMVYQI GGDILKTNFT DHDGLHVGIM GAYGYQDSKT HNKYTSYSSR GTVSGYTAGL 
901:	YSSWFQDEKE RTGLYMDAWL QYSWFNNTVK GDGLTGEKYS SKGITGALEA GYIYPTIRWT 
961:	AHNNIDNALY LNPQVQITRH GVKANDYIEH NGTMVTSSGG NNIQAKLGLR TSLISQSCID 
1021:	KETLRKFEPF LEVNWKWSSK QYGVIMNGMS NHQIGNRNVI ELKTGVGGRL ADNLSIWGNV 
1081:	SQQLGNNSYR DTQGILGVKY TF