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1.B.12.4.2
Autoexporter of temperature-sensitive hemagglutinin, a hemoglobin binding protease, Tsh/Hbp (1377 aas) (Jong and Luirink, 2008; Peterson et al., 2006). The pore of the Hbp TD is largely obstructed, but a variant that lacked one amino acid residue from the N-terminus showed the opening and closing of a channel comparable to what was reported for the TD of NalP. Hbp is processed by an autocatalytic intramolecular mechanism resulting in the stable docking of the α-helical plug in the barrel.

Accession Number:Q47692
Protein Name:Tsh/Hbp (1377 aas)
Length:1377
Molecular Weight:148227.00
Species:Escherichia coli [562]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell outer membrane1 / Multi-pass membrane protein2
Substrate Proteases

Cross database links:

RefSeq: YP_002539316.1    YP_444132.1   
Entrez Gene ID: 3853553    7379504   
Pfam: PF03797    PF02395   

Gene Ontology

GO:0009279 C:cell outer membrane
GO:0009986 C:cell surface
GO:0005576 C:extracellular region
GO:0016021 C:integral to membrane
GO:0042597 C:periplasmic space
GO:0005886 C:plasma membrane
GO:0004252 F:serine-type endopeptidase activity
GO:0009405 P:pathogenesis
GO:0006508 P:proteolysis

References (4)

[1] “Isolation and characterization of a gene involved in hemagglutination by an avian pathogenic Escherichia coli strain.”  Provence D.L.et.al.   8132344
[2] “Relationship between the Tsh autotransporter and pathogenicity of avian Escherichia coli and localization and analysis of the tsh genetic region.”  Dozois C.M.et.al.   10858231
[3] “Characterization of the avian pathogenic Escherichia coli hemagglutinin Tsh, a member of the immunoglobulin A protease-type family of autotransporters.”  Stathopoulos C.et.al.   9916089
[4] “Functional analysis of the Tsh autotransporter from an avian pathogenic Escherichia coli strain.”  Kostakioti M.et.al.   15385451
Structure:
3aeh     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
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FASTA formatted sequence
1:	MNRIYSLRYS AVARGFIAVS EFARKCVHKS VRRLCFPVLL LIPVLFSAGS LAGTVNNELG 
61:	YQLFRDFAEN KGMFRPGATN IAIYNKQGEF VGTLDKAAMP DFSAVDSEIG VATLINPQYI 
121:	ASVKHNGGYT NVSFGDGENR YNIVDRNNAP SLDFHAPRLD KLVTEVAPTA VTAQGAVAGA 
181:	YLDKERYPVF YRLGSGTQYI KDSNGQLTQM GGAYSWLTGG TVGSLSSYQN GEMISTSSGL 
241:	VFDYKLNGAM PIYGEAGDSG SPLFAFDTVQ NKWVLVGVLT AGNGAGGRGN NWAVIPLDFI 
301:	GQKFNEDNDA PVTFRTSEGG ALEWSFNSST GAGALTQGTT TYAMHGQQGN DLNAGKNLIF 
361:	QGQNGQINLK DSVSQGAGSL TFRDNYTVTT SNGSTWTGAG IVVDNGVSVN WQVNGVKGDN 
421:	LHKIGEGTLT VQGTGINEGG LKVGDGKVVL NQQADNKGQV QAFSSVNIAS GRPTVVLTDE 
481:	RQVNPDTVSW GYRGGTLDVN GNSLTFHQLK AADYGAVLAN NVDKRATITL DYALRADKVA 
541:	LNGWSESGKG TAGNLYKYNN PYTNTTDYFI LKQSTYGYFP TDQSSNATWE FVGHSQGDAQ 
601:	KLVADRFNTA GYLFHGQLKG NLNVDNRLPE GVTGALVMDG AADISGTFTQ ENGRLTLQGH 
661:	PVIHAYNTQS VADKLAASGD HSVLTQPTSF SQEDWENRSF TFDRLSLKNT DFGLGRNATL 
721:	NTTIQADNSS VTLGDSRVFI DKNDGQGTAF TLEEGTSVAT KDADKSVFNG TVNLDNQSVL 
781:	NINDIFNGGI QANNSTVNIS SDSAVLGNST LTSTALNLNK GANALASQSF VSDGPVNISD 
841:	AALSLNSRPD EVSHTLLPVY DYAGSWNLKG DDARLNVGPY SMLSGNINVQ DKGTVTLGGE 
901:	GELSPDLTLQ NQMLYSLFNG YRNIWSGSLN APDATVSMTD TQWSMNGNST AGNMKLNRTI 
961:	VGFNGGTSPF TTLTTDNLDA VQSAFVMRTD LNKADKLVIN KSATGHDNSI WVNFLKKPSN 
1021:	KDTLDIPLVS APEATADNLF RASTRVVGFS DVTPILSVRK EDGKKEWVLD GYQVARNDGQ 
1081:	GKAAATFMHI SYNNFITEVN NLNKRMGDLR DINGEAGTWV RLLNGSGSAD GGFTDHYTLL 
1141:	QMGADRKHEL GSMDLFTGVM ATYTDTDASA DLYSGKTKSW GGGFYASGLF RSGAYFDVIA 
1201:	KYIHNENKYD LNFAGAGKQN FRSHSLYAGA EVGYRYHLTD TTFVEPQAEL VWGRLQGQTF 
1261:	NWNDSGMDVS MRRNSVNPLV GRTGVVSGKT FSGKDWSLTA RAGLHYEFDL TDSADVHLKD 
1321:	AAGEHQINGR KDSRMLYGVG LNARFGDNTR LGLEVERSAF GKYNTDDAIN ANIRYSF