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1.B.12.4.3
Autotransporter of serine protease, EspP (with long N-terminal leader that prevents improper folding in the periplasm) (Szabady et al., 2005; Ieva et al., 2008). Energy for export is provided by the folding of the C-terminal domain (Peterson et al., 2010).

Accession Number:Q7BSW5
Protein Name:Putative exoprotein-precursor (Serine protease EspP)
Length:1300
Molecular Weight:141758.00
Species:Escherichia coli O157:H7 [83334]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell outer membrane1 / Multi-pass membrane protein2
Substrate Serine protease, EspP

Cross database links:

HEGENOM: HBG467630
RefSeq: NP_052685.1    YP_002756610.1    YP_325580.1   
Entrez Gene ID: 1789732    3683719    7701441   
Pfam: PF03797    PF02395   
KEGG: ece:L7020   

Gene Ontology

GO:0009279 C:cell outer membrane
GO:0009986 C:cell surface
GO:0005576 C:extracellular region
GO:0016021 C:integral to membrane
GO:0042597 C:periplasmic space
GO:0005886 C:plasma membrane
GO:0004252 F:serine-type endopeptidase activity
GO:0009405 P:pathogenesis
GO:0006508 P:proteolysis

References (6)

[1] “EspP, a novel extracellular serine protease of enterohaemorrhagic Escherichia coli O157:H7 cleaves human coagulation factor V.”  Brunder W.et.al.   9194704
[2] “The complete DNA sequence and analysis of the large virulence plasmid of Escherichia coli O157:H7.”  Burland V.et.al.   9722640
[3] “Complete nucleotide sequences of 93-kb and 3.3-kb plasmids of an enterohemorrhagic Escherichia coli O157:H7 derived from Sakai outbreak.”  Makino K.et.al.   9628576
[4] “The large plasmids of Shiga-toxin-producing Escherichia coli (STEC) are highly variable genetic elements.”  Brunder W.et.al.   10376815
[5] “Hydrophobic residues of the autotransporter EspP linker domain are important for outer membrane translocation of its passenger.”  Velarde J.J.et.al.   15151995
[6] “An unusual signal peptide facilitates late steps in the biogenesis of a bacterial autotransporter.”  Szabady R.L.et.al.   15615856
Structure:
2QOM   3SLJ   3SLO   3SLT   3SZE     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNKIYSLKYS HITGGLIAVS ELSGRVSSRA TGKKKHKRIL ALCFLGLLQS SYSFASQMDI 
61:	SNFYIRDYMD FAQNKGIFQA GATNIEIVKK DGSTLKLPEV PFPDFSPVAN KGSTTSIGGA 
121:	YSITATHNTK NHHSVATQNW GNSTYKQTDW NTSHPDFAVS RLDKFVVETR GATEGADISL 
181:	SKQQALERYG VNYKGEKKLI AFRAGSGVVS VKKNGRITPF NEVSYKPEML NGSFVHIDDW 
241:	SGWLILTNNQ FDEFNNIASQ GDSGSALFVY DNQKKKWVVA GTVWGIYNYA NGKNHAAYSK 
301:	WNQTTIDNLK NKYSYNVDMS GAQVATIENG KLTGTGSDTT DIKNKDLIFT GGGDILLKSS 
361:	FDNGAGGLVF NDKKTYRVNG DDFTFKGAGV DTRNGSTVEW NIRYDNKDNL HKIGDGTLDV 
421:	RKTQNTNLKT GEGLVILGAE KTFNNIYITS GDGTVRLNAE NALSGGEYNG IFFAKNGGTL 
481:	DLNGYNQSFN KIAATDSGAV ITNTSTKKSI LSLNNTADYI YHGNINGNLD VLQHHETKKE 
541:	NRRLILDGGV DTTNDISLRN TQLSMQGHAT EHAIYRDGAF SCSLPAPMRF LCGSDYVAGM 
601:	QNTEADAVKQ NGNAYKTNNA VSDLSQPDWE TGTFRFGTLH LENSDFSVGR NANVIGDIQA 
661:	SKSNITIGDT TAYIDLHAGK NITGDGFGFR QNIVRGNSQG ETLFTGGITA EDSTIVIKDK 
721:	AKALFSNYVY LLNTKATIEN GADVTTQSGM FSTSDISISG NLSMTGNPDK DNKFEPSIYL 
781:	NDASYLLTDD SARLVAKNKA SVVGDIHSTK SASIMFGHDE SDLSQLSDRT SKGLALGLLG 
841:	GFDVSYRGSV NAPSASATMN NTWWQLTGDS ALKTLKSTNS MVYFTDSANN KKFHTLTVDE 
901:	LATSNSAYAM RTNLSESDKL EVKKHLSGEN NILLVDFLQK PTPEKQLNIE LVSAPKDTNE 
961:	NVFKASKQTI GFSDVTPVIT TRETDDKITW SLTGYNTVAN KEATRNAAAL FSVDYKAFLN 
1021:	EVNNLNKRMG DLRDINGEAG AWARIMSGTG SASGGFSDNY THVQVGVDKK HELDGLDLFT 
1081:	GFTVTHTDSS ASADVFSGKT KSVGAGLYAS AMFDSGAYID LIGKYVHHDN EYTATFAGLG 
1141:	TRDYSTHSWY AGAEAGYRYH VTEDAWIEPQ AELVYGSVSG KQFAWKDQGM HLSMKDKDYN 
1201:	PLIGRTGVDV GKSFSGKDWK VTARAGLGYQ FDLLANGETV LRDASGEKRI KGEKDSRMLM 
1261:	SVGLNAEIRD NVRFGLEFEK SAFGKYNVDN AVNANFRYSF