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1.B.12.4.4
Autotransporter-1, Pet (serine protease; 1295 aas)) (Eslava et al., 1998Leyton et al., 2010).  The first stage of autotransporter folding determines whether subsequent translocation can deliver the N-terminal domain to its functional form on the bacterial cell surface. Paired conserved glycine-aromatic 'mortise and tenon' motifs join neighbouring beta-strands in the C-terminal barrel domain, and mutations within these motifs slow the rate and extent of passenger domain translocation to the surface of bacterial cell (Leyton et al. 2014).

Accession Number:O68900
Protein Name:Serine protease pet autotransporter
Length:1295
Molecular Weight:139769.00
Species:Escherichia coli O44:H18 (strain 042 / EAEC) [216592]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell outer membrane1 / Multi-pass membrane protein2
Substrate proteins

Cross database links:

Pfam: PF03797    PF02395   

Gene Ontology

GO:0009279 C:cell outer membrane
GO:0009986 C:cell surface
GO:0005576 C:extracellular region
GO:0016021 C:integral to membrane
GO:0042597 C:periplasmic space
GO:0005886 C:plasma membrane
GO:0004252 F:serine-type endopeptidase activity
GO:0009405 P:pathogenesis
GO:0006508 P:proteolysis

References (5)

[1] “Pet, an autotransporter enterotoxin from enteroaggregative Escherichia coli.”  Eslava C.et.al.   9632580
[2] “Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042.”  Chaudhuri R.R.et.al.   20098708
[3] “Cytoskeletal effects induced by pet, the serine protease enterotoxin of enteroaggregative Escherichia coli.”  Navarro-Garcia F.et.al.   10225873
[4] “Involvement of the enteroaggregative Escherichia coli plasmid-encoded toxin in causing human intestinal damage.”  Henderson I.R.et.al.   10496914
[5] “Plasmid-encoded toxin of enteroaggregative Escherichia coli is internalized by epithelial cells.”  Navarro-Garcia F.et.al.   11160002
Structure:
4OM9     

External Searches:

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Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNKIYSIKYS AATGGLIAVS ELAKKVICKT NRKISAALLS LAVISYTNII YAANMDISKA 
61:	WARDYLDLAQ NKGVFQPGST HVKIKLKDGT DFSFPALPVP DFSSATANGA ATSIGGAYAV 
121:	TVAHNAKNKS SANYQTYGST QYTQINRMTT GNDFSIQRLN KYVVETRGAD TSFNYNENNQ 
181:	NIIDRYGVDV GNGKKEIIGF RVGSGNTTFS GIKTSQTYQA DLLSASLFHI TNLRANTVGG 
241:	NKVEYENDSY FTNLTTNGDS GSGVYVFDNK EDKWVLLGTT HGIIGNGKTQ KTYVTPFDSK 
301:	TTNELKQLFI QNVNIDNNTA TIGGGKITIG NTTQDIEKNK NNQNKDLVFS GGGKISLKEN 
361:	LDLGYGGFIF DENKKYTVSA EGNNNVTFKG AGIDIGKGST VDWNIKYASN DALHKIGEGS 
421:	LNVIQAQNTN LKTGNGTVIL GAQKTFNNIY VAGGPGTVQL NAENALGEGD YAGIFFTENG 
481:	GKLDLNGHNQ TFKKIAATDS GTTITNSNTT KESVLSVNNQ NNYIYHGNVD GNVRLEHHLD 
541:	TKQDNARLIL DGDIQANSIS IKNAPLVMQG HATDHAIFRT TKTNNCPEFL CGVDWVTRIK 
601:	NAENSVNQKN KTTYKSNNQV SDLSQPDWET RKFRFDNLNI EDSSLSIARN ADVEGNIQAK 
661:	NSVINIGDKT AYIDLYSGKN ITGAGFTFRQ DIKSGDSIGE SKFTGGIMAT DGSISIGDKA 
721:	IVTLNTVSSL DRTALTIHKG ANVTASSSLF TTSNIKSGGD LTLTGATEST GEITPSMFYA 
781:	AGGYELTEDG ANFTAKNQAS VTGDIKSEKA AKLSFGSADK DNSATRYSQF ALAMLDGFDT 
841:	SYQGSIKAAQ SSLAMNNALW KVTGNSELKK LNSTGSMVLF NGGKNIFNTL TVDELTTSNS 
901:	AFVMRTNTQQ ADQLIVKNKL EGANNLLLVD FIEKKGNDKN GLNIDLVKAP ENTSKDVFKT 
961:	ETQTIGFSDV TPEIKQQEKD GKSVWTLTGY KTVANADAAK KATSLMSGGY KAFLAEVNNL 
1021:	NKRMGDLRDI NGEAGAWARI MSGTGSAGGG FSDNYTHVQV GADNKHELDG LDLFTGVTMT 
1081:	YTDSHAGSDA FSGETKSVGA GLYASAMFES GAYIDLIGKY VHHDNEYTAT FAGLGTRDYS 
1141:	SHSWYAGAEV GYRYHVTDSA WIEPQAELVY GAVSGKQFSW KDQGMNLTMK DKDFNPLIGR 
1201:	TGVDVGKSFS GKDWKVTARA GLGYQFDLFA NGETVLRDAS GEKRIKGEKD GRMLMNVGLN 
1261:	AEIRDNVRFG LEFEKSAFGK YNVDNAINAN FRYSF