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1.B.12.5.9
Autoexporter of lipase/esterase, EstA

Accession Number:O33407
Protein Name:EstA aka PA5112
Length:646
Molecular Weight:69609.00
Species:Pseudomonas aeruginosa [287]
Location1 / Topology2 / Orientation3: Cell outer membrane1 / Multi-pass membrane protein2
Substrate Esterases

Cross database links:

HEGENOM: HBG639316
RefSeq: NP_253799.1   
Entrez Gene ID: 878826   
Pfam: PF03797    PF00657   
BioCyc: PAER208964:PA5112-MONOMER   
KEGG: pae:PA5112   

Gene Ontology

GO:0009279 C:cell outer membrane
GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0004091 F:carboxylesterase activity
GO:0016298 F:lipase activity
GO:0006629 P:lipid metabolic process

References (2)

[1] “A novel lipolytic enzyme located in the outer membrane of Pseudomonas aeruginosa.”  Wilhelm S.et.al.   10559163
[2] “Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen.”  Stover C.K.et.al.   10984043
Structure:
3KVN     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MIRMALKPLV AACLLASLST APQAAPSPYS TLVVFGDSLS DAGQFPDPAG PAGSTSRFTN 
61:	RVGPTYQNGS GEIFGPTAPM LLGNQLGIAP GDLAASTSPV NAQQGIADGN NWAVGGYRTD 
121:	QIYDSITAAN GSLIERDNTL LRSRDGYLVD RARQGLGADP NALYYITGGG NDFLQGRILN 
181:	DVQAQQAAGR LVDSVQALQQ AGARYIVVWL LPDLGLTPAT FGGPLQPFAS QLSGTFNAEL 
241:	TAQLSQAGAN VIPLNIPLLL KEGMANPASF GLAADQNLIG TCFSGNGCTM NPTYGINGST 
301:	PDPSKLLFND SVHPTITGQR LIADYTYSLL SAPWELTLLP EMAHGTLRAY QDELRSQWQA 
361:	DWENWQNVGQ WRGFVGGGGQ RLDFDSQDSA ASGDGNGYNL TLGGSYRIDE AWRAGVAAGF 
421:	YRQKLEAGAK DSDYRMNSYM ASAFVQYQEN RWWADAALTG GYLDYDDLKR KFALGGGERS 
481:	EKGDTNGHLW AFSARLGYDI AQQADSPWHL SPFVSADYAR VEVDGYSEKG ASATALDYDD 
541:	QKRSSKRLGA GLQGKYAFGS DTQLFAEYAH EREYEDDTQD LTMSLNSLPG NRFTLEGYTP 
601:	QDHLNRVSLG FSQKLAPELS LRGGYNWRKG EDDTQQSVSL ALSLDF