TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


1.B.12.8.2
Fluffing protein (Flu) or antigen-43 (Ag-43; Ag43; also called YeeQ and YzzX). Processed proteolytically to the α- (soluble) and β- (membrane anchored) subunits; determines colony morphology and autoaggregation of E. coli K12 and many pathogenic strains (Henderson et al., 1997; Klemm et al. 2006).  May function in autotransporter processing.

Accession Number:P39180
Protein Name:Fluffing protein aka AG43
Length:1039
Molecular Weight:106825.00
Species:Escherichia coli [83333]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell outer membrane1 / Multi-pass membrane protein2
Substrate Protein virulence factor

Cross database links:

Genevestigator: P39180
EchoBASE: EB2550
EcoGene: EG12686
eggNOG: COG3468
HEGENOM: HBG467276
DIP: DIP-2892N
RefSeq: AP_002599.1    YP_026164.1   
Entrez Gene ID: 946540   
Pfam: PF03797   
BioCyc: EcoCyc:G7080-MONOMER   
KEGG: ecj:JW1982    eco:b2000   

Gene Ontology

GO:0009279 C:cell outer membrane
GO:0009986 C:cell surface
GO:0005576 C:extracellular region
GO:0016021 C:integral to membrane
GO:0042597 C:periplasmic space
GO:0005886 C:plasma membrane

References (7)

[1] “A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.”  Itoh T.et.al.   9097040
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005.”  Riley M.et.al.   16397293
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Purification and N-terminal sequence of the alpha subunit of antigen 43, a unique protein complex associated with the outer membrane of Escherichia coli.”  Caffrey P.et.al.   2661530
[6] “Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.”  Link A.J.et.al.   9298646
[7] “Antigen 43, a phase-variable bipartite outer membrane protein, determines colony morphology and autoaggregation in Escherichia coli K-12.”  Henderson I.R.et.al.   9103983

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MKRHLNTCYR LVWNHMTGAF VVASELARAR GKRGGVAVAL SLAAVTSLPV LAADIVVHPG 
61:	ETVNGGTLAN HDNQIVFGTT NGMTISTGLE YGPDNEANTG GQWVQDGGTA NKTTVTSGGL 
121:	QRVNPGGSVS DTVISAGGGQ SLQGRAVNTT LNGGEQWMHE GAIATGTVIN DKGWQVVKPG 
181:	TVATDTVVNT GAEGGPDAEN GDTGQFVRGD AVRTTINKNG RQIVRAEGTA NTTVVYAGGD 
241:	QTVHGHALDT TLNGGYQYVH NGGTASDTVV NSDGWQIVKN GGVAGNTTVN QKGRLQVDAG 
301:	GTATNVTLKQ GGALVTSTAA TVTGINRLGA FSVVEGKADN VVLENGGRLD VLTGHTATNT 
361:	RVDDGGTLDV RNGGTATTVS MGNGGVLLAD SGAAVSGTRS DGKAFSIGGG QADALMLEKG 
421:	SSFTLNAGDT ATDTTVNGGL FTARGGTLAG TTTLNNGAIL TLSGKTVNND TLTIREGDAL 
481:	LQGGSLTGNG SVEKSGSGTL TVSNTTLTQK AVNLNEGTLT LNDSTVTTDV IAQRGTALKL 
541:	TGSTVLNGAI DPTNVTLASG ATWNIPDNAT VQSVVDDLSH AGQIHFTSTR TGKFVPATLK 
601:	VKNLNGQNGT ISLRVRPDMA QNNADRLVID GGRATGKTIL NLVNAGNSAS GLATSGKGIQ 
661:	VVEAINGATT EEGAFVQGNR LQAGAFNYSL NRDSDESWYL RSENAYRAEV PLYASMLTQA 
721:	MDYDRIVAGS RSHQTGVNGE NNSVRLSIQG GHLGHDNNGG IARGATPESS GSYGFVRLEG 
781:	DLMRTEVAGM SVTAGVYGAA GHSSVDVKDD DGSRAGTVRD DAGSLGGYLN LVHTSSGLWA 
841:	DIVAQGTRHS MKASSDNNDF RARGWGWLGS LETGLPFSIT DNLMLEPQLQ YTWQGLSLDD 
901:	GKDNAGYVKF GHGSAQHVRA GFRLGSHNDM TFGEGTSSRA PLRDSAKHSV SELPVNWWVQ 
961:	PSVIRTFSSR GDMRVGTSTA GSGMTFSPSQ NGTSLDLQAG LEARVRENIT LGVQAGYAHS 
1021:	VSGSSAEGYN GQATLNVTF