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1.B.12.9.1
Autotransporter of N-terminal protease passenger domain that cleaves surface-localized virulence factors.  The 3-d structure is known (Oomen et al., 2004). The crystal structure of the NalP translocator domain revealed a 12 β-stranded transmembrane beta-barrel containing a central alpha-helix. The transmembrane beta-barrel is stable even in the absence of the alpha-helix. Removal of the helix results in an influx of water into the pore region, suggesting the helix acts as a 'plug' (Khalid and Sansom 2006). The dimensions of the pore fluctuate, but the NalP monomer is sufficient for the transport of the passenger domain in an unfolded or extended conformation (Khalid and Sansom 2006). NalP is subject to phase variation (Oldfield et al. 2013).

Accession Number:Q8GKS5
Protein Name:NalP
Length:1083
Molecular Weight:113640.00
Species:Neisseria meningitidis [487]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Protease, water, small molecules

Cross database links:

Pfam: PF03797    PF00082   

Gene Ontology

GO:0019867 C:outer membrane
GO:0004252 F:serine-type endopeptidase activity
GO:0006508 P:proteolysis

References (3)

[1] “In vivo expression of Neisseria meningitidis proteins homologous to the Haemophilus influenzae Hap and Hia autotransporters.”  van Ulsen P.et.al.   11750223
[2] “A Neisserial autotransporter NalP modulating the processing of other autotransporters.”  van Ulsen P.et.al.   14617158
[3] “Structure of the translocator domain of a bacterial autotransporter.”  Oomen C.J.et.al.   15014442
Structure:
1UYN   1UYO     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MRTTPTFPTK TFKPTAMALA VATTLSACLG GGGGGTSAPD FNAGGTGIGS NSRATTAKSA 
61:	AVSYAGIKNE MCKDRSMLCA GRDDVAVTDR DAKINAPPPP NLHTGDFPNP NDAYKNLINL 
121:	KPAIEAGYTG RGVEVGIVDT GESVGSISFP ELYGRKEHGY NENYKNYTAY MRKEAPEDGG 
181:	GKDIEASFDD EAVIETEAKP TDIRHVKEIG HIDLVSHIIG GRSVDGRPAG GIAPDATLHI 
241:	MNTNDGTKNE MMVAAIRNAW VKLGERGVRI VNNSFGTTSR AGTADLFQIA NSEEQYRQAL 
301:	LDYSGGDKTD EGIRLMQQSD YGNLSYHIRN KNMLFIFSTG NDAQAQPNTY ALLPFYEKDA 
361:	QKGIITVAGV DRSGEKFKRE MYGEPGTEPL EYGSNHCGIT AMWCLSAPYE ASVRFTRTNP 
421:	IQIAGTSFSA PIVTGTAALL LQKYPWMSND NLRTTLLTTA QDIGAVGVDS KFGWGLLDAG 
481:	KAMNGPASFP FGDFTADTKG TSDIAYSFRN DISGTGGLIK KGGSQLQLHG NNTYTGKTII 
541:	EGGSLVLYGN NKSDMRVETK GALIYNGAAS GGSLNSDGIV YLADTDQSGA NETVHIKGSL 
601:	QLDGKGTLYT RLGKLLKVDG TAIIGGKLYM SARGKGAGYL NSTGRRVPFL SAAKIGQDYS 
661:	FFTNIETDGG LLASLDSVEK TAGSEGDTLS YYVRRGNAAR TASAAAHSAP AGLKHAVEQG 
721:	GSNLENLMVE LDASESSATP ETVETAAADR TDMPGIRPYG ATFRAAAAVQ HANAADGVRI 
781:	FNSLAATVYA DSTAAHADMQ GRRLKAVSDG LDHNGTGLRV IAQTQQDGGT WEQGGVEGKM 
841:	RGSTQTVGIA AKTGENTTAA ATLGMGRSTW SENSANAKTD SISLFAGIRH DAGDIGYLKG 
901:	LFSYGRYKNS ISRSTGADEH AEGSVNGTLM QLGALGGVNV PFAATGDLTV EGGLRYDLLK 
961:	QDAFAEKGSA LGWSGNSLTE GTLVGLAGLK LSQPLSDKAV LFATAGVERD LNGRDYTVTG 
1021:	GFTGATAATG KTGARNMPHT RLVAGLGADV EFGNGWNGLA RYSYAGSKQY GNHSGRVGVG 
1081:	YRF