TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


1.B.14.1.2
FhuA ferrichrome (also albomycin and rifamycin; Colicin M; Microcin J25; Phage T5) receptor (transports phage T1, T5 and φ80 DNA across the outer membrane, dependent on DcrA (SdaC; TC #2.A.42.2.1) and DcrB) (Forms a complex with and acts with TonB and FhuD (the periplasmic binding receptor (3.A.1.14.3) to deliver siderophore to FhuD (Carter et al., 2006; Braun et al., 2009)).  Deletion of the 160-residue cork domain and five large extracellular loops converted this non-conductive, monomeric, 22-stranded beta-barrel protein into a large-conductance protein pore (Wolfe et al. 2015).

Accession Number:P06971
Protein Name:FhuA aka TONA aka B0150
Length:747
Molecular Weight:82182.00
Species:Escherichia coli [83333]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell outer membrane1 / Multi-pass membrane protein2
Substrate Ferrichrome, ALbomycin, Rifamycin, Colicin M, Microcin J25, Phage T1, Phage T5, Phage Phi-80

Cross database links:

Genevestigator: P06971
EchoBASE: EB0298
EcoGene: EG10302
eggNOG: COG1629
HEGENOM: HBG564417
DIP: DIP-9602N
RefSeq: AP_000811.1    NP_414692.1   
Entrez Gene ID: 944856   
Pfam: PF07715    PF00593   
Drugbank: Drugbank Link   
BioCyc: EcoCyc:EG10302-MONOMER    ECOL168927:B0150-MONOMER   
KEGG: ecj:JW0146    eco:b0150   

Gene Ontology

GO:0009279 C:cell outer membrane
GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0048037 F:cofactor binding
GO:0005506 F:iron ion binding
GO:0004872 F:receptor activity
GO:0015343 F:siderophore-iron transmembrane transporter ...
GO:0015643 F:toxin binding
GO:0046790 F:virion binding
GO:0009597 P:detection of virus
GO:0046718 P:entry of virus into host cell
GO:0006826 P:iron ion transport
GO:0015891 P:siderophore transport

References (10)

[1] “Protein fusions of beta-galactosidase to the ferrichrome-iron receptor of Escherichia coli K-12.”  Coulton J.W.et.al.   3079747
[2] “Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region.”  Fujita N.et.al.   8202364
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Nucleotide sequence of the fhuC and fhuD genes involved in iron (III) hydroxamate transport: domains in FhuC homologous to ATP-binding proteins.”  Burkhardt R.et.al.   2823072
[6] “Energy-coupled transport through the outer membrane of Escherichia coli small deletions in the gating loop convert the FhuA transport protein into a diffusion channel.”  Braun V.et.al.   7515827
[7] “FhuA, a transporter of the Escherichia coli outer membrane, is converted into a channel upon binding of bacteriophage T5.”  Bonhivers M.et.al.   8617231
[8] “Siderophore-mediated iron transport: crystal structure of FhuA with bound lipopolysaccharide.”  Ferguson A.D.et.al.   9856937
[9] “Transmembrane signaling across the ligand-gated FhuA receptor: crystal structures of free and ferrichrome-bound states reveal allosteric changes.”  Locher K.P.et.al.   9865695
[10] “Crystal structure of the antibiotic albomycin in complex with the outer membrane transporter FhuA.”  Ferguson A.D.et.al.   10850805
Structure:
1BY3   1BY5   1FCP   1FI1   1QFF   1QFG   1QJQ   1QKC   2FCP   2GRX   [...more]

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MARSKTAQPK HSLRKIAVVV ATAVSGMSVY AQAAVEPKED TITVTAAPAP QESAWGPAAT 
61:	IAARQSATGT KTDTPIQKVP QSISVVTAEE MALHQPKSVK EALSYTPGVS VGTRGASNTY 
121:	DHLIIRGFAA EGQSQNNYLN GLKLQGNFYN DAVIDPYMLE RAEIMRGPVS VLYGKSSPGG 
181:	LLNMVSKRPT TEPLKEVQFK AGTDSLFQTG FDFSDSLDDD GVYSYRLTGL ARSANAQQKG 
241:	SEEQRYAIAP AFTWRPDDKT NFTFLSYFQN EPETGYYGWL PKEGTVEPLP NGKRLPTDFN 
301:	EGAKNNTYSR NEKMVGYSFD HEFNDTFTVR QNLRFAENKT SQNSVYGYGV CSDPANAYSK 
361:	QCAALAPADK GHYLARKYVV DDEKLQNFSV DTQLQSKFAT GDIDHTLLTG VDFMRMRNDI 
421:	NAWFGYDDSV PLLNLYNPVN TDFDFNAKDP ANSGPYRILN KQKQTGVYVQ DQAQWDKVLV 
481:	TLGGRYDWAD QESLNRVAGT TDKRDDKQFT WRGGVNYLFD NGVTPYFSYS ESFEPSSQVG 
541:	KDGNIFAPSK GKQYEVGVKY VPEDRPIVVT GAVYNLTKTN NLMADPEGSF FSVEGGEIRA 
601:	RGVEIEAKAA LSASVNVVGS YTYTDAEYTT DTTYKGNTPA QVPKHMASLW ADYTFFDGPL 
661:	SGLTLGTGGR YTGSSYGDPA NSFKVGSYTV VDALVRYDLA RVGMAGSNVA LHVNNLFDRE 
721:	YVASCFNTYG CFWGAERQVV ATATFRF