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1.B.17.3.5
CusC outer membrane exporter of copper ion, Cu+, and silver ion Ag+.  The crystal structure of CusC is known (Lei et al. 2013) providing evidnce concerning the folding mechanism giving rise to the channel.

Accession Number:P77211
Protein Name:Cation efflux system protein cusC
Length:457
Molecular Weight:50270.00
Species: [83333]
Location1 / Topology2 / Orientation3: Cell outer membrane1 / Lipid-anchor2
Substrate Ag+, Cu+

Cross database links:

Genevestigator: P77211
EchoBASE: EB3984
EcoGene: EG14233
eggNOG: COG1538
HEGENOM: HBG759011
DIP: DIP-9347N
RefSeq: AP_001217.1    NP_415104.1   
Entrez Gene ID: 946288   
Pfam: PF02321   
BioCyc: EcoCyc:G6320-MONOMER    ECOL168927:B0572-MONOMER   
KEGG: ecj:JW0561    eco:b0572   

Gene Ontology

GO:0009279 C:cell outer membrane
GO:0005886 C:plasma membrane
GO:0005507 F:copper ion binding
GO:0005375 F:copper ion transmembrane transporter activity
GO:0008289 F:lipid binding
GO:0005515 F:protein binding
GO:0006878 P:cellular copper ion homeostasis
GO:0060003 P:copper ion export
GO:0010273 P:detoxification of copper ion
GO:0010272 P:response to silver ion

References (9)

[1] “Identification and characterization of an Escherichia coli invasion gene locus, ibeB, required for penetration of brain microvascular endothelial cells.”  Huang S.-H.et.al.   10225861
[2] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Identification of a copper-responsive two-component system on the chromosome of Escherichia coli K-12.”  Munson G.P.et.al.   11004187
[6] “Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography.”  Fountoulakis M.et.al.   10493123
[7] “The independent cue and cus systems confer copper tolerance during aerobic and anaerobic growth in Escherichia coli.”  Outten F.W.et.al.   11399769
[8] “The product of the ybdE gene of the Escherichia coli chromosome is involved in detoxification of silver ions.”  Franke S.et.al.   11283292
[9] “Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli.”  Franke S.et.al.   12813074
Structure:
3PIK   4K34   4K7K   4K7R     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSPCKLLPFC VALALTGCSL APDYQRPAMP VPQQFSLSQN GLVNAADNYQ NAGWRTFFVD 
61:	NQVKTLISEA LVNNRDLRMA TLKVQEARAQ YRLTDADRYP QLNGEGSGSW SGNLKGNTAT 
121:	TREFSTGLNA SFDLDFFGRL KNMSEAERQN YLATEEAQRA VHILLVSNVA QSYFNQQLAY 
181:	AQLQIAEETL RNYQQSYAFV EKQLLTGSSN VLALEQARGV IESTRSDIAK RQGELAQANN 
241:	ALQLLLGSYG KLPQAQTVNS DSLQSVKLPA GLSSQILLQR PDIMEAEHAL MAANANIGAA 
301:	RAAFFPSISL TSGISTASSD LSSLFNASSG MWNFIPKIEI PIFNAGRNQA NLDIAEIRQQ 
361:	QSVVNYEQKI QNAFKEVADA LALRQSLNDQ ISAQQRYLAS LQITLQRARA LYQHGAVSYL 
421:	EVLDAERSLF ATRQTLLDLN YARQVNEISL YTALGGG