about | faq
TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


1.C.38.1.2
Sticholysin I (cytolysin ST1 or STII or StiII) (Alvarez et al., 2009). Pore formation goes through a dimer intermediate and then generates the active octamer. Disrupting the key hydrophobic interaction between V60 and F163 (FraC numbering scheme) in the oligomerization interface of FraC, equinatoxin II (EqtII) and sticholysin II (StII) impairs the pore formation activity (Mesa-Galloso et al. 2016). Sticholysin II-mediated cytotoxicity may involve the activation of regulated intracellular responses that anticipates cell death (Soto et al. 2018).

Accession Number:P81662
Protein Name:STCH1 aka STCH
Length:176
Molecular Weight:19391.00
Species:Stoichactis helianthus (Carribean sea anemone) (Stichodactyla helianthus) [6123]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Secreted1
Substrate small molecules

Cross database links:

Pfam: PF06369   

Gene Ontology

GO:0005576 C:extracellular region
GO:0042151 C:nematocyst
GO:0046930 C:pore complex
GO:0015267 F:channel activity
GO:0006812 P:cation transport
GO:0019835 P:cytolysis
GO:0019836 P:hemolysis by symbiont of host erythrocytes
GO:0009405 P:pathogenesis
GO:0046931 P:pore complex assembly

References (4)

[1] “Primary structure of two cytolysin isoforms from Stichodactyla helianthus differing in their hemolytic activity.”  Huerta V.et.al.   11306138
[2] “Purification and characterization of two hemolysins from Stichodactyla helianthus.”  Lanio M.E.et.al.   10978735
[3] “Properties of St I and St II, two isotoxins isolated from Stichodactyla helianthus: a comparison.”  Martinez D.et.al.   11478962
[4] “Structural and functional characterization of a recombinant sticholysin I (rSt I) from the sea anemone Stichodactyla helianthus.”  Pazos F.et.al.   17067649
Structure:
2KS4     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence 
1:	SELAGTIIDG ASLTFEVLDK VLGELGKVSR KIAVGIDNES GGTWTALNAY FRSGTTDVIL 
61:	PEVVPNTKAL LYSGRKSSGP VATGAVAAFA YYMSNGNTLG VMFSVPFDYN WYSNWWDVKI 
121:	YPGKRRADQG MYEDMYYGNP YRGDNGWYQK NLGYGLRMKG IMTSAGEAKM QIKISR