TCDB is operated by the Saier Lab Bioinformatics Group
TCIDNameDomainKingdom/PhylumProtein(s)
*1.C.39.1.1









Uncharacterized MACPF homologue of 1153 aas

Eukaryota
Dictyosteliida
UP of Dictyostelium fasciculatum (Slime mold)
*1.C.39.1.2









Uncharacterized protein of 1277 aas

Eukaryota
Dictyosteliida
UP of Polysphondylium pallidum (Cellular slime mold)
*1.C.39.1.3









Uncharacterized protein of 1216 aas

Eukaryota
Dictyosteliida
UP of Acytostelium subglobosum
*1.C.39.1.4









Uncharacterized protein of 1151 aas

Eukaryota
Dictyosteliida
UP of Polysphondylium pallidum
*1.C.39.2.1









Perforin 1 precursor; targets viruses, bacteria and cancer cells (McCormack et al. 2013). It is produced by cytotoxic T lymphocytes and natural killer cells and has been expressed, purified and studied in insect cells (Naneh et al. 2015).

Eukaryota
Metazoa
Perforin of Rattus norvegicus
*1.C.39.2.2









Uncharacterized protein of 429 aas

Eukaryota
Metazoa
UP of Astyanax mexicanus (Blind cave fish) (Astyanax fasciatus mexicanus)
*1.C.39.2.3









Performin 1-like protein of 545 aas, Prf1

Eukaryota
Metazoa
Prf1 of Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
*1.C.39.2.4









Uncharacterized protein of 481 aas

Eukaryota
Metazoa
UP of Latimeria chalumnae (West Indian ocean coelacanth)
*1.C.39.2.5









Performin 1, Pfn1, of 587 aa

Eukaryota
Metazoa
Pfn1 of Carassius auratus langsdorfii (Japanese silver crucian carp)
*1.C.39.2.6









MACPF domain-containing protein (342aas)

Eukaryota
Intramacronucleata
MACPF proteins of Tetrahymena thermophila (Q23MJ4)
*1.C.39.2.7









Duplicated MACPF protein (681aas) The first half resembles 1.C.39.6.2 more than the second half.

Eukaryota
Intramacronucleata
MACPF protein of Tetrahymena thermophila (Q23I78)
*1.C.39.2.8









Perforin 1, PRF1 of 555 aas.  Plays a key role in secretory granule-dependent cell death and in defense against virus-infected and neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores (Law et al. 2010). Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. Perforin gene mutations contribute to hereditary cancer predisposition (Chaudhry et al. 2016).

Eukaryota
Metazoa
Perforin of Homo sapiens
*1.C.39.3.1









Pore-forming, membrane attack, complement component 8, α-polypeptide precursor; C8α-MACPF (structure solved to 2.5 Å resolution; Hadders et al., 2007; Rosado et al., 2007).  β-Hairpins in C8α and C9 play a direct role in MAC membrane penetration and pore formation (Weiland et al. 2014).

Eukaryota
Metazoa
C8α-MACPF of Homo sapiens (2QQH_A) (P07357)
*1.C.39.3.2









Complement component 7

Eukaryota
Metazoa
Complement component 7 of Xenopus laevis (Q6INM0)
*1.C.39.3.3









Perforin 2; (perforin-2; perforin2;MPEG1) complement component C6; targets phagocytic and some non phagocytic cells (McCormack et al. 2013).  Expressed constitutively in phagocytes and inducibly in parenchymal tissue-forming cells. It is a transmembrane protein of cytosolic vesicles, derived from multiple organelles that translocate to and fuse with bacterium containing vesicles. Subsequently, Perforin-2 polymerizes and forms large clusters of 100 Å pores in the bacterial surface with Perforin-2 cleavage products present in the bacteria. Perforin-2 is also required for the bactericidal activity of reactive oxygen and nitrogen species as well as hydrolytic enzymes (McCormack et al. 2015).

Eukaryota
Metazoa
Perforin 2 of Homo sapiens
*1.C.39.3.4









MACPF protein, terminal complement component, TCC-like of 585 aas.

Eukaryota
Metazoa
TCC-like protein of Halocynthia roretzi (Sea squirt) (Cynthia roretzi)
*1.C.39.3.5









Complement protein C9; targets bacteria (McCormack et al. 2013).  β-Hairpins in C8α and C9 play a direct role in MAC membrane penetration and pore formation (Weiland et al. 2014).

Eukaryota
Metazoa
C9 of Equus caballus
*1.C.39.3.6









Complement protein C9.  β-Hairpins in C8α and C9 play a direct role in MAC membrane penetration and pore formation (Weiland et al. 2014).

Eukaryota
Metazoa
C9 of Fugu rubripes
*1.C.39.4.1









Chain A, MACPF perforin-like protein, Plu-MACPF (structure solved to 2.0 Å resolution; Rosado et al., 2007).

Bacteria
Proteobacteria
Plu-MACPF of Photorhabdus luminescens (2QP2_A) (Q7N6X0)
*1.C.39.4.2









MACPF protein (453 aas) 

Bacteria
Cyanobacteria
MACPF protein of Trichodesmium erythraeum (Q117U3)
*1.C.39.4.3









Hypothetical Protein (588 aas)

Bacteria
Proteobacteria
HP of Marinomonas sp. MED121 (A3YG19)
*1.C.39.4.4









Putative perforin of 409 aas

Archaea
Euryarchaeota
Putative perforin of Halorubrum kocurii
*1.C.39.4.5









Uncharacterized protein of 684 aas

Eukaryota
Viridiplantae
UP of Selaginella moellendorffii
*1.C.39.4.6









Uncharacterized protein of 1085 aas

Bacteria
Tenericutes
UP of Acholeplasma palmae
*1.C.39.5.1









MACPF homologue

Eukaryota
Metazoa
MACPF homologue of Branchiostoma floridae (C3YI39)
*1.C.39.5.2









MACPF homologue

Eukaryota
Metazoa
MACPF homologue of Nematostella vectensis (A7RF41)
*1.C.39.5.3









MACPF homologue

Eukaryota
Metazoa
MACPF homologue of Branchiostoma floridae (C3Z435)
*1.C.39.5.4









Protein of 1305 aas with an N-terminal MACPF domain and C-terminal extracellular cystine-rich furin-like (Fu-sup), fucolectin (tocylectin; discoidin; FTP; a fucose-binding lectin) and cystine-rich scavenger receptor (SRCR; extracellular protein-protein interaction) domains (in this order, N- to C-terminus).

Eukaryota
Metazoa
MACPF protein of Branchiostoma floridae (Florida lancelet) (Amphioxus)
*1.C.39.5.5









Uncharacterized protein of 632 aas

Bacteria
Proteobacteria
UP of Pseudomonas thivervalensis
*1.C.39.6.1









Sporozoite protein with MAC/Perforin domain (Homologous to Erylysin B) of 810 aas.  Interacts and breaches host cell membranes (Tavares et al. 2014).  CDC/MACPF proteins contain a characteristic four-stranded beta-sheet that is flanked by two alpha-helical bundles, which unfold to form two transmembrane beta-hairpins. Apicomplexan parasites express CDC/MACPFs termed perforin-like proteins (PLPs). Wade and Tweten 2015 present insights into the assembly and regulation of the Apicomplexan CDC (ApiMACPF) molecular pore-forming mechanisms, necessary for osmotically driven rupture of the parasitophorous vacuole and host cell membrane, and cell traversal by these parasites.

Eukaryota
Apicomplexa
MACPF protein of Plasmodium knowlesi (B3L016)
*1.C.39.6.2









Perforin-like protein, PLP1, of 1150 aas (Tavares et al. 2014).

Eukaryota
Apicomplexa
PLP1 of Toxoplasma gondii
*1.C.39.6.3









MACPF protein 

Eukaryota
Apicomplexa
MACPF protein of Theileria parva (Q4MYP3)
*1.C.39.6.4









MACPF domain-containing protein (420aas)

Eukaryota
Apicomplexa
MACPF protein of Babesia bovis (A7AT97)
*1.C.39.7.1









MAC/Perforin domain protein

Eukaryota
Intramacronucleata
MACPF domain protein of Tetrahymena thermophila (Q23QV5)
*1.C.39.7.2









Uncharacterized protein of 1040 aas

Eukaryota
Ichthyosporea
UP of Capsaspora owczarzaki
*1.C.39.7.3









The MAC/Perforin domain containing protein of 861 aas

Eukaryota
Intramacronucleata
MACPF protein of Oxytricha trifallax
*1.C.39.7.4









Apextrin of 853 aas

Eukaryota
Metazoa
Apextrin of Acropora millepora (Staghorn coral)
*1.C.39.7.5









Putative uncharacterized phospholipase D endonuclease of 487 aas

Bacteria
Proteobacteria
UP of Myxococcus fulvus
*1.C.39.8.1









MACPF-Hemopexin protein.  The MACPF domain forms pores in the membrane while the hemopexin domain fuctions as a heme scavenging domain, protecting the cell against heme toxicity (Mehta and Reddy 2015).

Bacteria
Proteobacteria
Hemopexin of Plesiocystis pacifica (A6G7F3)
*1.C.39.8.2









The MACPF protein homologue with hemopexin-like C-terminal repeats

Bacteria
Proteobacteria
MACPF protein of Beggiotoa sp. PS (A7BVI9)
*1.C.39.8.3









Photopexin a/b-like protein of 347 aas.

Bacteria
Proteobacteria
Photopexin of Photorhabdus temperat
*1.C.39.9.1









MACPF homologue

Eukaryota
Fungi
MACPF homologue of Postia placenta (B8PKX3)
*1.C.39.9.2









Uncharacterized MACPF protein of 446 aas.  The MACPF domain includes residues 120 - 320.

Eukaryota
Fungi
UP of Emericella nidulans (Aspergillus nidulans)
*1.C.39.9.3









Uncharacterized MACPF protein of 483 aas

Eukaryota
Fungi
UP of Fusarium oxysporum f. sp. vasinfectum
*1.C.39.9.4









Uncharacterized MACPF protein of 420 aas

Eukaryota
Fungi
UP of Trichophyton verrucosum
*1.C.39.9.5









Uncharacterized protein of 461 aas

Eukaryota
Fungi
UP of Ceriporiopsis subvermispora (White-rot fungus)
*1.C.39.10.1









Sea anemone toxin, AvTX-60A, of 498aas (Oshiro et al., 2004).

Eukaryota
Metazoa
AvTX-60A of Actineria villosa (Q76DT2)
*1.C.39.10.2









MACPF-containing actinoporin of 488 aas, PsTX60B (Frazão et al. 2012).

Eukaryota
Metazoa
PsTX60B of Phyllodiscus semoni (Night anemone)
*1.C.39.10.3









Uncharacterized protein of 449 aas

UP of Selaginella moellendorffii (Spikemoss)
*1.C.39.10.4









Uncharacterized protein of 474 aas

Eukaryota
Metazoa
UP of Nematostella vectensis (Starlet sea anemone)
*1.C.39.11.1









MACPF protein (610aas)

Eukaryota
Viridiplantae
MACPF protein of Medicago truncatula (Q1SKW8)
*1.C.39.11.2









MACPF protein (615aas) 

Eukaryota
Viridiplantae
MACPF protein of Populus trichocarpa (B9GNC9)
*1.C.39.11.3









The constitutively activated cell death 1 protein (CAD1) of 561 aas (Morita-Yamamuro et al. 2005; Tsutsui et al. 2006Tsutsui et al. 2006).

Eukaryota
Viridiplantae
CAD1 of Arabidopsis thaliana
*1.C.39.11.4









The Necrotic Spotted Lesions 1 (NSL1) protein of 612 aas (Noutoshi et al. 2006).

Eukaryota
Viridiplantae
NSL1 of Arabidopsis thaliana
*1.C.39.12.1









MACPF protein (809aas)

Bacteria
Chlamydiae/Verrucomicrobia group
MACPF protein of Chlamydia muridarum (Q9PKN4)
*1.C.39.12.2









MACPF homologue (411aas)

Bacteria
Chlamydiae/Verrucomicrobia group
MACPF homologue of Chlamydophila pneumoniae (Q9Z908)
*1.C.39.12.3









MACPF protein, CT153 of 810 aas.  Mediates interactions with host cell membranes and organelles, and plays a role in intracellular development (Taylor and Nelson 2014).

Bacteria
Chlamydiae/Verrucomicrobia group
CT153 protein of Chlamydia trachomatis
*1.C.39.12.4









MACPF domain protein of 834 aas

Bacteria
Chlamydiae/Verrucomicrobia group
MACPF protein of Chlamydia psittaci
*1.C.39.13.1









Hypothetical protein (470aas)

Bacteria
Bacteroidetes/Chlorobi group
HP of Bacteroides thetaiotaomicron (Q8A335)
*1.C.39.13.2









Hypothetical protein (372aas) 

Bacteria
Bacteroidetes/Chlorobi group
HP of Bacteroides fragilis (Q64VU4)
*1.C.39.13.3









Hypothetical Protein (486 aas)

Bacteria
Bacteroidetes/Chlorobi group
HP of Bacteroides fragilis (Q64W10)
*1.C.39.13.4









MACPF protein. The structure is known (Xu et al., 2010).

Bacteria
Bacteroidetes/Chlorobi group
MACPF protein of Bacteroides thetaiotaomicron (Q8A267)
*1.C.39.13.5









Uncharacterized protein

Bacteria
Bacteroidetes/Chlorobi group
UP of Paraprevotella xylaniphila
*1.C.39.14.1









Macrophage-expressed gene 1 protein, Mpeg1 of 716 aas.  Pore formation has been demonstrated in target bacteria (McCormack et al. 2013).

Eukaryota
Metazoa
Mpeg1 of Homo sapiens
*1.C.39.14.2









MACPF domain protein, Mpeg of 742 aas.  This protein is found in late endosomes.  Its MACPF domain exhibits anti-bacterial activity against Gram - and Gram + bacteria.  It's synthesis is stimulated following infection with Vibrio alginolyticus (He et al. 2011).

Eukaryota
Metazoa
Mpeg of Crassostrea gigas (Pacific oyster) (Crassostrea angulata)
*1.C.39.14.3









Mpeg1 of 728 aas.  Contains a cytolytic MACPF domain.  Expressed in up to 8x increase in hematocytes and epipodia samples after exposure to heat killed Vibrio anguilarum (Kemp and Coyne 2011).

Eukaryota
Metazoa
Mpeg1 of Haliotis midae (perlemoen abalone)
*1.C.39.14.4









Mpeg1 of 718 aas (Benard et al. 2014).

Eukaryota
Metazoa
Mpeg1 of Danio rerio (Zebrafish) (Brachydanio rerio)
*1.C.39.15.1









Torso-like protein, Tsl of 353 aas and containing a MACPF domain.  Possible ligand that binds to the torso receptor. Implicated in a receptor tyrosine kinase signaling pathway that specifies differentialtion and terminal cell fate (Martin et al. 1994; Savant-Bhonsale and Montell 1993; Johnson et al. 2013; Mineo et al. 2015).

Eukaryota
Metazoa
Tsl of Drosophila melanogaster (Fruit fly)
*1.C.39.15.2









Uncharacterized Torso-like protein of 271 aas

Eukaryota
Metazoa
Torso-like protein of Daphnia pulex (Water flea)
*1.C.39.16.1









Uncharacterized protein of 784 aas

Eukaryota
Fungi
UP of Penicillium marneffei
*1.C.39.16.2









Uncharacterized protein of 795 aas

Eukaryota
Fungi
UP of Cladophialophora psammophila
*1.C.39.17.1









The BMP/retinoic acid-inducible neural-specific protein 1, BRINP1 (DBC1, DBCCR1, FAM5A), protein of 761 aas. Inhibits cell proliferation by negative regulation of the G1/S transition and mediates cell death which is not of the classical apoptotic type while regulating expression of components of the plasminogen pathway (Wright et al. 2004; Nishiyama et al. 2001; Louhelainen et al. 2006).

Eukaryota
Metazoa
BRINP-1 of Homo sapiens
*1.C.39.17.2









BRINP-2 (BRINP2, FAM5B) of 783 aas.  Inhibits neuronal cell proliferation by negative regulation of the cell cycle transition.

Eukaryota
Metazoa
BRINP-2 of Homo sapiens