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1.C.76 The Pore-forming Maculatin Peptide (Maculatin) Family

Maculatins exist in four isoforms (1.1, 1.2, 2.1 and 3.1). They form pores in lipid bilayers (Ambroggio et al., 2005). Each of these small peptides consists of a single α-helical TMS per polypeptide chain which oligomerize to produce pores in biological membranes. These can cause leakage and thus kill the cell.

Maculatin forms an ensemble of structurally diverse temporarily functional low-oligomeric pores, which mimic integral membrane protein channels in structure (Wang et al. 2016). These pores continuously form and dissociate in the membrane. Membrane permeabilization is dominated by hexa-, hepta- and octamers, which conduct water, ions and small dyes. Pores form by consecutive addition of individual helices to a transmembrane helix or helix bundle. The diversity of the pore architectures-formed by a single sequence-may be a key feature in preventing bacterial resistance and could explain why sequence-function relationships in AMPs remain elusive (Wang et al. 2016).

This family belongs to the: Cecropin Superfamily.

References associated with 1.C.76 family:

Ambroggio, E.E., F. Separovic, J.H. Bowie, G.D. Fidelio, and L.A. Bagatolli. (2005). Direct visualization of membrane leakage induced by the antibiotic peptides: maculatin, citropin, and aurein. Biophys J. 89: 1874-1881. 15994901
Fernandez DI., Gehman JD. and Separovic F. (2009). Membrane interactions of antimicrobial peptides from Australian frogs. Biochim Biophys Acta. 1788(8):1630-8. 19013126
Mechler, A., S. Praporski, K. Atmuri, M. Boland, F. Separovic, and L.L. Martin. (2007). Specific and selective peptide-membrane interactions revealed using quartz crystal microbalance. Biophys. J. 93: 3907-3916. 17704161
Rozek, T., K.L. Wegener, J.H. Bowie, I.N. Olver, J.A. Carver, J.C. Wallace, and M.J. Tyler. (2000). The antibiotic and anticancer active aurein peptides from the Australian Bell Frogs Litoria aurea and Litoria raniformis the solution structure of aurein 1.2. Eur J Biochem 267: 5330-5341. 10951191
Wang, G., Y. Li, and X. Li. (2005). Correlation of three-dimensional structures with the antibacterial activity of a group of peptides designed based on a nontoxic bacterial membrane anchor. J. Biol. Chem. 280: 5803-5811. 15572363
Wang, Y., C.H. Chen, D. Hu, M.B. Ulmschneider, and J.P. Ulmschneider. (2016). Spontaneous formation of structurally diverse membrane channel architectures from a single antimicrobial peptide. Nat Commun 7: 13535. 27874004