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1.C.95 The Pore-forming ESAT-6 Protein (ESAT-6) Family

The ESX-1 secretion system (TC# 9.A.25) plays a critical role in virulence of M. tuberculosis and M. marinum. Virulent M marinum is able to escape from the Mycobacterium-containing vacuole (MCV) into the host cell cytosol, polymerize actin, and spread from cell to cell. ESX-1 plays an essential role in M. marinum escape from the MCV.

Smith et al. (2008) provided evidence that M. marinum induces membrane pores approximately 4.5 nm in diameter, and this activity correlates with ESAT-6 secretion. Purified ESTAT-6, but not the other ESX-1-secreted proteins, is able to cause dose-dependent pore formation in host cell membranes.

The reaction catalyzed for ESAT-6 is:

molecules (vacuoles) molecules (cytoplasm)

References associated with 1.C.95 family:

Smith, J., J. Manoranjan, M. Pan, A. Bohsali, J. Xu, J. Liu, K.L. McDonald, A. Szyk, N. LaRonde-LeBlanc, and L.Y. Gao. (2008). Evidence for pore formation in host cell membranes by ESX-1-secreted ESAT-6 and its role in Mycobacterium marinum escape from the vacuole. Infect. Immun. 76: 5478-5487. 18852239