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1.D.63 The Peptide-based Nanopore (PepNP) Family 

Re-engineering nature's molecules provides a strategy to obtain nanopores. Lella and Mahalakshmi 2016 reported a peptide engineering strategy through sequence reversal, which was applied onto the first transmembrane peptide of a mycobacteriophage membranoporin protein holin. They redesigned the membrane rupture property of this peptide to form nanopore ion channels with specific properties. The structural characterization and electrophysiology properties of a library of 28 engineered peptides with differing ion channel behaviors was reported. They identified key residues, charge distributions, and hydropathy indices of the peptides that contribute to the channel characteristics.

References associated with 1.D.63 family:

Lella, M. and R. Mahalakshmi. (2016). Engineering a Transmembrane Nanopore Ion Channel from a Membrane Breaker Peptide. J Phys Chem Lett 2298-2303. [Epub: Ahead of Print] 27257735