1.D.63 The Peptide-based Nanopore (PepNP) Family
Re-engineering nature's molecules provides a strategy to obtain nanopores. Lella and Mahalakshmi 2016 reported a peptide engineering strategy through sequence reversal, which was applied onto the first transmembrane peptide of a mycobacteriophage membranoporin protein holin. They redesigned the membrane rupture property of this peptide to form nanopore ion channels with specific properties. The structural characterization and electrophysiology properties of a library of 28 engineered peptides with differing ion channel behaviors was reported. They identified key residues, charge distributions, and hydropathy indices of the peptides that contribute to the channel characteristics.