TCDB is operated by the Saier Lab Bioinformatics Group
TCIDNameDomainKingdom/PhylumProtein(s)
*1.E.14.1.1









LrgA holin-like protein (Bayles, 2003; Yang et al., 2005; Ranjit et al. 2011).  Functions in biofilm formation (Ranjit et al. 2011).  Calcium-chelating alizarin and other anthraquinones inhibit biofilm formation and modulate the expression of the cid/lrg genes (encoding the holin/antiholin system) (Lee et al. 2016).

Bacteria
Firmicutes
LrgA of Staphylococcus aureus (147 aas; gbU52961)
*1.E.14.1.2









Holin, CidA (Bayles, 2003; Yang et al., 2005; Ranjit et al. 2011).  Functions in biofilm formation in part by mediating release of cytoplasmic DNA during cell lysis to contribute to the biofilm matrix (Ranjit et al. 2011; Fischer et al. 2013).  This protein contributes to cell lysis of dying cells (Patton et al. 2005).  Calcium-chelating alizarin and other anthraquinones inhibit biofilm formation and modulate the expression of the cid/lrg genes (encoding the holin/antiholin system) (Lee et al. 2016).

Bacteria
Firmicutes
CidA of Staphylococcus aureus (P60646)
*1.E.14.1.3









Marine hydrolase exporter (MHE) (Desvaux et al. 2005).

Bacteria
Fusobacteria
MHE of Fusobacterium mortiferum (C3WD05)
*1.E.14.1.4









4 TMS LrgA putative holin/anti-holin

Bacteria
Proteobacteria
LrgA of E. coli (F4V3T5)
*1.E.14.1.5









4 TMS murein hydrolase exporter, LrgA-like protein

Archaea
Euryarchaeota
LrgA-like protein of Thermococcus gammatolerans (C5A1Q2)
*1.E.14.1.6









4 TMS Hypothetical protein (HP)

Archaea
Euryarchaeota
HP of Pyrococcus furiosus (Q8TZY1)
*1.E.14.1.7









4 TMS LrgA-like protein 

Bacteria
Chloroflexi
LrgA-like protein of Chloroflexus aggregans (B8GAY6)
*1.E.14.1.8









4 TMS holin protein 

Bacteria
Firmicutes
Holin of Pediococcus acidilactici (D2EIF4)
*1.E.14.1.9









CidA holin-like protein

Bacteria
Proteobacteria
CidA of E. coli (E7UC95)
*1.E.14.1.10









LrgA holin, involved in biofilm formation, oxidative stress and competence for DNA transfer. Regulated at the transcriptional level by the two component regulatory system, LytST (Ahn et al. 2012).

Bacteria
Firmicutes
LrgA of Streptococcus mutants
*1.E.14.1.11









LrgA-type holin of 136 aas and 4 TMSs

Bacteria
Deinococcus-Thermus
Putative holin of Deinococcus deserti
*1.E.14.1.12









LrgA family holin of 118 aas and 4 TMSs

Bacteria
Firmicutes
Holin of Bacillus toyonensis
*1.E.14.1.13









Holin of 114 aas and 3 TMSs

Bacteria
Bacteroidetes/Chlorobi group
Holin of Capnocytophaga ochracea
*1.E.14.1.14









LrgA family holin of 129 aas and 4 TMSs

Bacteria
Firmicutes
Holin of Exiguobacterium sibiricum
*1.E.14.1.15









LrgA paralogue of 120 aas

Bacteria
Firmicutes
LrgA of Streptococcus mutans
*1.E.14.1.16









Putative holin of 128 aas and 4 TMSs, CidA or YwbH.  Functions in acetic acid promotion of biofilm formation (Chen et al. 2015).  Increases the activity of extracellular murein hydrolases probably by mediating their export via hole formation. May be inhibited by LrgB/YwbG (2.A.122.1.5).

Bacteria
Firmicutes
CidA of Bacillus subtilis
*1.E.14.1.17









Putative holin (or anti-holin) of 146 aas and 4 TMSs, YsbA or LrgA.  YsbA is important for acetic acid induced biofilm formation (Chen et al. 2015) and for pyruvate utilization (van den Esker et al. 2016). van den Esker et al. 2016 proposed that YsbA is a pyruvate transporter.  Thus, there is some question about the function of this protein and other members of this family.

Bacteria
Firmicutes
YsbA of Bacillus subtilis
*1.E.14.2.1









Putative archaeal holin of 145 aas and 4 TMSs

Archaea
Euryarchaeota
Putative holin of Methanocaldococcus (Methanococcus) vulcanius
*1.E.14.2.2









Putative archaeal holin of 145 aas and 4 TMSs

Archaea
Euryarchaeota
Holin of Methanocaldococcus vulcanius
*1.E.14.2.3









Putative holin of 194 aas and 5 TMSs

Archaea
Euryarchaeota
Putative holin of Methanocaldococcus sp.