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1.E.2 The Lambda Holin S (λ Holin) Family

Lambda holin S has 3 TMS with the N-terminus in the periplasm and the C-terminus in the cytoplasm. It is the prototype for class I holins. Its 107 codon sequence encodes two proteins with opposing functions, the holin, S105, and the holin inhibitor, S107, synthesized as a result of a distinct translational initiation event.  It's first TMS (TMS1) is required for holin function but not for antiholin function (White et al. 2010).

The latter protein is a 2-amino acid extension of the former protein due to a different translational initiation start site (M1-K2-M3 vs. M3). The cationic amino acid at position 2 is largely responsible for the inhibiting effect of S107. The ratio of S105 to S107 influences the timing of phage lambda-induced cell lysis. The highly hydrophilic C-terminal domains of holins (e.g., lambda S105) have been shown to be localized cytoplasmically and serve as regulatory domains. Like the N-terminal 2 amino acid extension in S107, they influence the timing of lysis by a charge dependent mechanism (Gründling et al. 2000).

Expression of holin S at a precisely scheduled time after phage infection terminates respiration and allows release of a muralytic enzyme, endolysin, that hydrolyzes the cell wall. Point mutations in the S gene that prevent lethality alter TMSs 1 and 2 and the connecting loop. TMS 2 is particularly important for function. A three-step mechanism (monomer → dimer → oligomeric pore) has been proposed for assembly of the pore. S105 (holin) and S107 (inhibitor) form an abortive dimer. Only when S105 production exceeds that of S107 (which occurs at a specific developmental time), do functional holes appear in the bacterial cell membrane (Graschopf and Bläsi 1999).

Holins control the length of the infection cycle of tailed phages (the Caudovirales) by oligomerizing to form lethal holes in the cytoplasmic membrane at a time dictated by their primary structures. Savva et al. (2008) used electron microscopy and single-particle analysis to characterize structures formed by the bacteriophage lambda holin (S105) in vitro. In non-ionic or mild zwitterionic detergents, purified S105, but not the lysis-defective variant S105A52V, formed rings of at least two size classes, the most common having inner and outer diameters of 8.5 and 23 nm respectively, and containing approximately 72 S105 monomers. The height of these rings, 4 nm, closely matches the thickness of the lipid bilayer. The central channel is of unprecedented size for channels formed by integral membrane proteins, consistent with the non-specific nature of holin-mediated membrane permeabilization. S105, present in detergent-solubilized rings and in inverted membrane vesicles, showed similar sensitivities to proteolysis and cysteine-specific modification, suggesting that the rings are representative of the lethal holes formed by S105 to terminate the infection cycle and initiate lysis (Savva et al., 2008).

A homologue of λ holin S from the lysogenic Xenorhabdus nematophila, hol-1 (TC #1.E.2.1.4), has been shown to be a functional holin. When cloned into wild-type E. coli, it causes hemolysis due to the release of the SheA hemolysin (Brillard et al., 2003). Another holin (phage H-19B holin) is encoded by a gene associated with the Shiga-like toxin I gene of E. coli (Neely and Friedman, 1998). Thus, it appears that holins can export various toxins as well as autolysins.

The holes caused by S105 have an average diameter of 340 nm, and some exceeding 1 microm. Most cells exhibit only one irregular hole, randomly positioned in the membrane, irrespective of its size (Dewey et al., 2010).  During λ infection, S105 accumulates harmlessly in the membrane until it forms a single irregular hole, releasing the endolysin from the cytoplasm, resulting in lysis within seconds.  Using a functional S105-GFP fusion, it was demonstrated that the protein accumulates uniformly in the membrane, and then within 1 minute, it fomrs aggregates at the time of lethality.  Thus, like bacteriorhodopsin, the protein accumulates until it reaches a critical concentration for nucleation (White et al. 2011). 

This family belongs to the: Holin Superfamily III.

References associated with 1.E.2 family:

Agu, C.A., R. Klein, J. Lengler, F. Schilcher, W. Gregor, T. Peterbauer, U. Bläsi, B. Salmons, W.H. Günzburg, and C. Hohenadl. (2007). Bacteriophage-encoded toxins: the λ-holin protein causes caspase-independent non-apoptotic cell death of eukaryotic cells. Cell Microbiol 9: 1753-1765. 17346308
Barenboim, M., C.Y. Chang, F.D. Hajj, and R. Young. (1999). Characterization of the dual start motif of a class II holin gene. Mol. Microbiol. 32: 715-727. 10361276
Bläsi, U., P. Fraisl, C.Y. Chang, N. Zhang, and R. Young. (1999). The C-terminal sequence of the λholin constitutes a cytoplasmic regulatory domain. J. Bacteriol. 181: 2922-2929. 10217787
Brillard, J., M.-H. Boyer-Giglio, N. Boemare, and A. Givaudan. (2003). Holin locus characterisation from lysogenic Xenorhabdus nematophila and its involvement in Escherichia coli SheA haemolytic phenotype. FEMS Microbiol. Lett. 218: 107-113. 12583905
Dewey, J.S., C.G. Savva, R.L. White, S. Vitha, A. Holzenburg, and R. Young. (2010). Micron-scale holes terminate the phage infection cycle. Proc. Natl. Acad. Sci. USA 107: 2219-2223. 20080651
Dover, J.A., A.R. Burmeister, I.J. Molineux, and K.N. Parent. (2016). Evolved Populations of Shigella flexneri Phage Sf6 Acquire Large Deletions, Altered Genomic Architecture, and Faster Life Cycles. Genome Biol Evol 8: 2827-2840. 27497318
Graschopf, A. and U. Bläsi. (1999). Functional assembly of the lambda S holin requires periplasmic localization of its N-terminus. Arch. Microbiol. 172: 31-39. 10398749
Graschopf, A. and U. Bläsi. (1999). Molecular function of the dual-start motif in the λS holin. Mol. Microbiol. 33: 569-582. 10417647
Gründling, A., D.L. Smith, U. Bläsi, and R. Young. (2000). Dimerization between the holin and holin inhibitor of phage λ. J. Bacteriol. 182: 6075-6081. 11029427
Gründling, A., U. Bläsi, and R. Young. (2000). Biochemical and genetic evidence for three transmembrane domains in the class I holin, lambda S. J. Biol. Chem. 275: 769-776. 10625606
Gründling, A., U. Bläsi, and R. Young. (2000). Genetic and biochemical analysis of dimer and oligomer interactions of the λS holin. J. Bacteriol. 182: 6082-6090. 11029428
Neely, M.N. and D.I. Friedman. (1998). Functional and genetic analysis of regulatory regions of coliphage H-19B: location of shiga-like toxin and lysis genes suggest a role for phage functions in toxin release. Mol. Microbiol. 28: 1255-1267. 9680214
Savva, C.G., J.S. Dewey, J. Deaton, R.L. White, D.K. Struck, A. Holzenburg, and R. Young. (2008). The holin of bacteriophage lambda forms rings with large diameter. Mol. Microbiol. 69: 784-793. 18788120
White, R., S. Chiba, T. Pang, J.S. Dewey, C.G. Savva, A. Holzenburg, K. Pogliano, and R. Young. (2011). Holin triggering in real time. Proc. Natl. Acad. Sci. USA 108: 798-803. 21187415
White, R., T.A. Tran, C.A. Dankenbring, J. Deaton, and R. Young. (2010). The N-terminal transmembrane domain of lambda S is required for holin but not antiholin function. J. Bacteriol. 192: 725-733. 19897658