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1.H.1.1.9
Claudin 15 (with a cation selective paracellular channel (Angelow et al., 2008).  Claudin-15 is highly expressed in the intestine where it forms efficient Na+ channels and Cl- barriers. The permeation process of Na+, K+, and Cl- ions inside a refined structural model of a claudin-15 paracellular channel was investigated using all-atom molecular dynamics simulations in a double-bilayer (Alberini et al. 2018). The channel allows the passage of the two physiological cations while excluding chloride with 30x selectivity. These features are generated by the action of several acidic residues, in particular, the ring of D55 residues which is located at the narrowest region of the pore, in correspondence with the energy minimum for cations and the peak for chloride. Claudin-15 thus regulates tight junction selectivity by invoking the experimentally determined role of the acidic residues (Alberini et al. 2018). Water and small cations can pass through the channel, but larger cations, such as tetramethylammonium, do not (Samanta et al. 2018).

Accession Number:P56746
Protein Name:Cldn15
Length:228
Molecular Weight:24356.00
Species:Homo sapiens [9606]
Number of TMSs:4
Location1 / Topology2 / Orientation3: Cell junction1 / Multi-pass membrane protein2
Substrate cations

Cross database links:

Genevestigator: P56746
eggNOG: prNOG06918
HEGENOM: HBG716859
RefSeq: NP_055158.1   
Entrez Gene ID: 24146   
Pfam: PF00822   
KEGG: hsa:24146   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005923 C:tight junction
GO:0042802 F:identical protein binding
GO:0005198 F:structural molecule activity
GO:0016338 P:calcium-independent cell-cell adhesion

References (2)

[1] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[2] “Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.”  Mayya V.et.al.   19690332

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSMAVETFGF FMATVGLLML GVTLPNSYWR VSTVHGNVIT TNTIFENLWF SCATDSLGVY 
61:	NCWEFPSMLA LSGYIQACRA LMITAILLGF LGLLLGIAGL RCTNIGGLEL SRKAKLAATA 
121:	GALHILAGIC GMVAISWYAF NITRDFFDPL YPGTKYELGP ALYLGWSASL ISILGGLCLC 
181:	SACCCGSDED PAASARRPYQ APVSVMPVAT SDQEGDSSFG KYGRNAYV