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2.A.1.8.17
Nitrate/nitrite transporter, NarK2, of 468 aas and 12 TMSs. The narK1 and narK2 genes are located in an operon, narK1K2GHJI, with the structural genes for the nitrate reductase complex.  Utilizing an isogenic narK1 mutant, a narK2 mutant, and a narK1K2 double mutant, Sharma et al. 2006 explored the effect on growth under denitrifying conditions. While the ΔnarK1::Gm mutant was only slightly affected, but both the ΔnarK2::Gm and double mutants exhibited poor nitrate-dependent, anaerobic growth although all three strains had wild-type levels of nitrate reductase activity. Nitrate uptake measurements showed that NarK2 has most of the activity.  E. coli narK rescued both mutants.

Accession Number:Q9HXD4
Protein Name:Nitrite extrusion protein 2
Length:468
Molecular Weight:50612.00
Species:Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) [208964]
Number of TMSs:12
Substrate

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FASTA formatted sequence
1:	MGMIANTQLK AARGPLLLDW RPEDPEFWAR NGKRIASRNL WISIPALLLA FAVWMIWSTV 
61:	TVRLNSAGFA FSNDQLFLLA ALPSISGATL RVFYSFMVPI FGGRRWTALS TASMLIPCIW 
121:	LGFAVQDPST PYWVFALIAL LCGFGGGNFA SSMSNISFFY PKSQQGTALG LNAGLGNLGV 
181:	SVMQFAVPLA VAGGLFGAFG GEPQQLGDGG RLWLQNAGWI WVPFIAVVSV AAWFGMNDIA 
241:	DAKASFRDQA VIFKRKHNWL MCWLYVATFG SFIGFSAGFA MLSKTQFPDV NPLQYAFLGP 
301:	LVGALSRPLG GWLADKFGGA RITLWNYALM IGGVFAVIAF LPGDGGQGNF FGFLGAFILL 
361:	FFFAGIGNGS TFRMIPVIFR NEWARRVRDG QADEAGAARN ASMESAAVIG FSAAIGAFGG 
421:	FFIPKAFGTS LHMTGSAEGA LYVFIAYYLS CIVATWWWYA RKGAESPC