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2.A.17.3.17
Transporter for glucosinolates (aliphatic but not indole glucosinolates such as 4-methylthiobutyl glucosinolate, major defence compounds, translocated to seeds on maturation) as well as gibberellic acid and jasmonoyl-L-isoleucine, GTR1 or NPF2.10, of 636 aas and 12 TMSs (Nour-Eldin et al. 2012; Ishimaru et al. 2017). Regulated at the transcriptional level, but also postranslationally.  Dimerization of GTR1, possibly induced by dephosphorylation of a Thr residue, regulates its plasma membrane localization, leading to increased transport of glucosinolates and gibberellic acid (Ishimaru et al. 2017). Homologues have been found and characterized in Chinese kale (Jiang et al. 2019).

Accession Number:Q944G5
Protein Name:Probable peptide/nitrate transporter At3g47960
Length:636
Molecular Weight:71040.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate 4-methylthiobutyl glucosinolate, jasmonoyl-L-isoleucine, gibberellic acid, Alkyl glucosinolates

Cross database links:

Genevestigator: Q944G5
eggNOG: NOG259627
HEGENOM: HOG000237399
Entrez Gene ID: 823951   
Pfam: PF00854   
KEGG: ath:AT3G47960   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005215 F:transporter activity
GO:0006857 P:oligopeptide transport

References (6)

[1] “Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.”  Salanoubat M.et.al.   11130713
[2] “Empirical analysis of transcriptional activity in the Arabidopsis genome.”  Yamada K.et.al.   14593172
[3] “Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry.”  Nuehse T.S.et.al.   14506206
[4] “Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database.”  Nuehse T.S.et.al.   15308754
[5] “Nitrate transporters and peptide transporters.”  Tsay Y.F.et.al.   17481610
[6] “The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal from the xylem sap and mediates cadmium tolerance.”  Li J.Y.et.al.   20501909

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKSRVILNHR DRRDKNHNNN NTNHYTQVDT MERKPLEVEP STTTTNTDVV DSFEEEQRKI 
61:	VYRGWKVMPF IIGNETFEKL GIIGTLSNLL VYLTSVFNLK SYTAATIINA FSGTINFGTF 
121:	IAAFLCDTYF GRYKTLSVAV IACFLGSFVI LLTAAIPSLH PVACGNKISC EGPSVGQILF 
181:	LLMGLGFLVV GAGGIRPCNL AFGADQFNPK SESGKKGINS FFNWYFFTFT FAQIISLTAV 
241:	VYIQSNVSWT IGLIIPVALM FLACVIFFAG DRLYVKVKAS GSPLAGIARV IAAAIKKRGL 
301:	KPVKQPWVNL YNHIPSNYAN TTLKYTDQFR FLDKAAIMTP EEKLNSDGTA SDPWKLCTLQ 
361:	QVEEVKCIVR VIPIWFASTI YYLAITIQMT YPVFQALQSD RRLGSGGFRI PAATYVVFLM 
421:	TGMTVFIIFY DRVLVPSLRR VTGLETGISL LQRIGAGFTF AIMSLLVSGF IEERRRNFAL 
481:	TKPTLGMAPR TGEISSMSAL WLIPQLTLAG IAEAFAAIGQ MEFYYKQFPE NMKSFAGSIF 
541:	YVGAGVSSYL ASFLISTVHR TTAHSPSGNW LAEDLNKAKL DYFYFMLTGL MVVNMAYFLL 
601:	MARWYRYKGG NDEDITEIET NEEETKQQQL QDKNSV