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2.A.29.32.3
The Entamoeba transmembrane mitosomal protein of 30 kDa (ETMP30) of 260 aas and 5 equally distantly spaced TMSs. Its loss results in a defect in growth and partial elongation of mitosomes (Santos et al. 2019). The aerobic mitochondrion had undergone evolutionary diversification, most notable among lineages of anaerobic protists. Entamoeba is one of the genera of parasitic protozoans that lack canonical mitochondria, and instead possess mitochondrion-related organelles (MROs), specifically, mitosomes. Entamoeba mitosomes exhibit functional reduction and divergence, most exemplified by the organelle's inability to produce ATP and synthesize iron-sulfur clusters. Instead, this organelle is capable of sulfate activation, which has been linked to amoebic stage conversion (Santos et al. 2019). Colocalization of hemagglutinin (HA)-tagged ETMP30 with the mitosomal marker, adenosine-5'-phosphosulfate kinase. Mitosomal membrane localization was indicated by immunoelectron microscopy analysis. Transcriptional gene silencing successfully repressed RNA expression by 60%, and led to a defect in growth and partial elongation of mitosomes. Immunoprecipitation of ETMP30 from ETMP30-HA-expressing transformant using anti-HA antibody pulled down one interacting protein of 126 kDa. Protein sequencing by mass spectrometry revealed this protein as a cation-transporting P-type ATPase, previously reported to localize to vacuolar compartments/Golgi-like structures, hinting at a possible mitosome-vacuole/Golgi contact site (Santos et al. 2019). Colocalization of hemagglutinin (HA)-tagged ETMP30 with the mitosomal marker, adenosine-5'-phosphosulfate kinase. Mitosomal membrane localization was indicated by immunoelectron microscopy analysis. Transcriptional gene silencing successfully repressed RNA expression by 60%, and led to a defect in growth and partial elongation of mitosomes. Immunoprecipitation of ETMP30 from ETMP30-HA-expressing transformant using anti-HA antibody pulled down one interacting protein of 126 kDa. Protein sequencing by mass spectrometry revealed this protein as a cation-transporting P-type ATPase, previously reported to localize to vacuolar compartments/Golgi-like structures, hinting at a possible mitosome-vacuole/Golgi contact site (Santos et al. 2019Santos et al. 2019).

Accession Number:C4MAE5
Protein Name:Uncharacterized protein
Length:260
Molecular Weight:29385.00
Species:Entamoeba histolytica [5759]
Number of TMSs:5
Substrate

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FASTA formatted sequence
1:	MFSQYLSYVG GTLTQFIIEK PITTPFQAAL TCSQMNGLST WSNMSFLARK NSLFKSLPYS 
61:	ILNAIPLTVS EQLAIRLLLT QKERKTHKIS LCKQLAITSL STITSFPFTY LEIVNRTHCC 
121:	SDINLKDIKY AIPRPLNIPI IPLYWNILRN ITYSTSHSFT SLKLQNIKCF SSNSLIKINA 
181:	ISSMVGTLLS YPFDVLLSRS IHNYSTGFSP SILTHGICTQ LLINVSLSSA YGYLAEKYYE 
241:	KHSLKRSVFR SIKSIFFASQ