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2.A.3.10.7
Asn/Gln permease

Accession Number:P25376
Protein Name:Agp1 aka YCL025C aka YCL25C
Length:633
Molecular Weight:69671.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Asparagine, Glutamine

Cross database links:

Genevestigator: P25376
eggNOG: fuNOG07978
HEGENOM: HBG492579
DIP: DIP-4973N
RefSeq: NP_009905.3   
Entrez Gene ID: 850333   
Pfam: PF00324   
KEGG: sce:YCL025C   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015193 F:L-proline transmembrane transporter activity
GO:0005515 F:protein binding
GO:0006865 P:amino acid transport
GO:0055085 P:transmembrane transport

References (13)

[1] “The complete DNA sequence of yeast chromosome III.”  Oliver S.G.et.al.   1574125
[2] “The Saccharomyces cerevisiae YCC5 (YCL025c) gene encodes an amino acid permease, Agp1, which transports asparagine and glutamine.”  Schreve J.L.et.al.   9573211
[3] “Cysteine uptake by Saccharomyces cerevisiae is accomplished by multiple permeases.”  During-Olsen L.et.al.   10467005
[4] “Substrate specificity and gene expression of the amino-acid permeases in Saccharomyces cerevisiae.”  Regenberg B.et.al.   10654085
[5] “Amino acid signaling in Saccharomyces cerevisiae: a permease-like sensor of external amino acids and F-Box protein Grr1p are required for transcriptional induction of the AGP1 gene, which encodes a broad-specificity amino acid permease.”  Iraqui I.et.al.   9891035
[6] “Genetic and biochemical analysis of the yeast plasma membrane Ssy1p-Ptr3p-Ssy5p sensor of extracellular amino acids.”  Forsberg H.et.al.   11154269
[7] “Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii.”  Brachat S.et.al.   12844361
[8] “Global analysis of protein expression in yeast.”  Ghaemmaghami S.et.al.   14562106
[9] “Yeast Agp2p and Agp3p function as amino acid permeases in poor nutrient conditions.”  Schreve J.L.et.al.   14697254
[10] “The external amino acid signaling pathway promotes activation of Stp1 and Uga35/Dal81 transcription factors for induction of the AGP1 gene in Saccharomyces cerevisiae.”  Abdel-Sater F.et.al.   15126393
[11] “Global analysis of protein palmitoylation in yeast.”  Roth A.F.et.al.   16751107
[12] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258
[13] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSSSKSLYEL KDLKNSSTEI HATGQDNEIE YFETGSNDRP SSQPHLGYEQ HNTSAVRRFF 
61:	DSFKRADQGP QDEVEATQMN DLTSAISPSS RQAQELEKNE SSDNIGANTG HKSDSLKKTI 
121:	QPRHVLMIAL GTGIGTGLLV GNGTALVHAG PAGLLIGYAI MGSILYCIIQ ACGEMALVYS 
181:	NLTGGYNAYP SFLVDDGFGF AVAWVYCLQW LCVCPLELVT ASMTIKYWTT SVNPDVFVII 
241:	FYVLVITINI FGARGYAEAE FFFNCCKILM MTGFFILGII IDVGGAGNDG FIGGKYWHDP 
301:	GAFNGKHAID RFKGVAATLV TAAFAFGGSE FIAITTAEQS NPRKAIPGAA KQMIYRILFL 
361:	FLATIILLGF LVPYNSDQLL GSTGGGTKAS PYVIAVASHG VRVVPHFINA VILLSVLSMA 
421:	NSSFYSSARL FLTLSEQGYA PKVFSYIDRA GRPLIAMGVS ALFAVIAFCA ASPKEEQVFT 
481:	WLLAISGLSQ LFTWTAICLS HLRFRRAMKV QGRSLGELGF KSQTGVWGSA YACIMMILIL 
541:	IAQFWVAIAP IGEGKLDAQA FFENYLAMPI LIALYVGYKV WHKDWKLFIR ADKIDLDSHR 
601:	QIFDEELIKQ EDEEYRERLR NGPYWKRVVA FWC