TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.3.12.1
The plasma membrane polyamine (putrescine, spermidine):H+ uptake symporter, LmPOT1 (inhibited by pentamidine and protonophores) (Hasne and Ullmann, 2005)

Accession Number:Q5C8V6
Protein Name:Polyamine transporter (Amino acid permease, putative)
Length:803
Molecular Weight:84779.00
Species:Leishmania major [5664]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Golgi apparatus membrane1 / Multi-pass membrane protein2
Substrate Putrescine, Spermidine, H+

Cross database links:

HEGENOM: HBG521022
RefSeq: XP_001687631.1   
Entrez Gene ID: 5650231   
Pfam: PF00324   
KEGG: lma:LmjF14.0320   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0015171 F:amino acid transmembrane transporter activity
GO:0006865 P:amino acid transport
GO:0055085 P:transmembrane transport

References (2)

[1] “Identification and characterization of a polyamine permease from the protozoan parasite Leishmania major.”  Hasne M.P.et.al.   15632173
[2] “The genome of the kinetoplastid parasite, Leishmania major.”  Ivens A.C.et.al.   16020728

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MRHARERSGA TDGAHAGDGD LVHASSQIKD VVSVNGISLG CGGIEDEKLA TAANGKSVRV 
61:	RRNVEVIFHP ELRRTKEVIR RPEWVRRAGE CVAHRGSLST IALFGIMFAN CVGGGYGFED 
121:	GIGSAGPLIT LIVCGILPWM WAFPTGLAVA ELSTAVPSNS GVLMWTNAAF PPFMSFMCIL 
181:	ATIFITFIGN ATYPNLTAEY AQQLGNLKLA PVAGVKVGVV ALCCILNCVG VEIVGSSSII 
241:	LCAITILPFT LLTVIQLFSK GFNRAVLYVD VKKVNWAEFF SILSWNYANI ENAGAVVEEV 
301:	ANPRRALPKA MMMLMLSTYV GYVMPMLAGV SAMGVDQDYS KWKAGHWPEV AKVIAGDWLK 
361:	YMLFAGALLS GIGFTLTSMC CTSRLLAGMG TMQMFPKKVS RVIGYYHPRL GTPIPAIFIN 
421:	SLVTLVFSVS MEFTSVVSLC QSIYCLRMLL IYASLVKMRI DYPNLPRPYA LPFSTCTTAL 
481:	VLLPAAAFSL MASIVSAMTS LGIGMALVGF VVVGSGVSWL YCRIFARNGF QGVIVQCEVS 
541:	SGDDAEAGAA DSANDSALSE GIFYADEERN SGEPVDDLLL GILPMPLAAP GPLASTVLPR 
601:	GSEVSSSQWH VRHGMTDRDA IGSQAGEEMI GMEYALQDGP STDIMVVNSS ATAHALGSST 
661:	AVCSPTSSFH TYRVACGVET TMRHRHRARS HGAGGGGGGA TNSGNYGPAV SGEESGEDPD 
721:	ITPAEHSGGG FTLSATGLPG QVLSTSPPQL ILSPQQVLLN VPAAGSWSSS RSPTPASGPR 
781:	SPILTSAINM STTRKPPPPP TSL