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2.A.3.4.1
The single high affinity plasma membrane choline transporter of 563 aas and 12 TMSs. Expression of the CTR/HNM1 gene in wild-type cells is regulated by phospholipid precursors, inositol and choline, but no such effect is seen in cells bearing mutations in the phospholipid regulatory genes INO2, INO4 and OPI1. There is no regulation by INO2 and OPI1 in the absence of a conserved decamer motif. However constructs lacking this sequence are still controlled by INO4. Other substrates of the choline permease, i.e., ethanolamine, nitrogen mustard and nitrogen half mustard do not regulate expression of CTR/HNM1 (Li and Brendel 1993). Exposing cells to increasing levels of choline results in two different regulatory mechanisms. Initial exposure to choline results in a rapid decrease in Hnm1-mediated transport activity, whereas chronic exposure results in Hnm1 degradation through an endocytic mechanism that depends on the ubiquitin ligase Rsp5 and the casein kinase 1 redundant pair Yck1/Yck2 (Fernández-Murray et al. 2013).

Accession Number:P19807
Protein Name:Ctr aka Hnm1 aka CTR1 aka YGL077C
Length:563
Molecular Weight:62056.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Choline

Cross database links:

Genevestigator: P19807
eggNOG: fuNOG04280
HEGENOM: HBG327463
DIP: DIP-7965N
RefSeq: NP_011438.1   
Entrez Gene ID: 852803   
Pfam: PF00324   
KEGG: sce:YGL077C   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0015171 F:amino acid transmembrane transporter activity
GO:0015220 F:choline transmembrane transporter activity
GO:0034228 F:ethanolamine transmembrane transporter acti...
GO:0015653 F:glycine betaine:hydrogen symporter activity
GO:0042802 F:identical protein binding
GO:0015871 P:choline transport
GO:0034229 P:ethanolamine transport
GO:0031460 P:glycine betaine transport
GO:0055085 P:transmembrane transport

References (5)

[1] “Primary structure of the yeast choline transport gene and regulation of its expression.”  Nikawa J.et.al.   2203793
[2] “Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII.”  Rieger M.et.al.   9290212
[3] “The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.”  Tettelin H.et.al.   9169869
[4] “Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.”  Hu Y.et.al.   17322287
[5] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258

External Searches:

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSIRNDNASG GYMQPDQSSN ASMHKRDLRV EEEIKPLDDM DSKGAVAADG EVHLRKSFSL 
61:	WSILGVGFGL TNSWFGISTS MVAGISSGGP MMIVYGIIIV ALISICIGTS LGELSSAYPH 
121:	AGGQFWWSLK LAPPKYKRFA AYMCGSFAYA GSVFTSASTT LSVATEVVGM YALTHPEFIP 
181:	KRWHIFVCFE LLHLFLMFFN CYGKSLPIIS SSSLYISLLS FFTITITVLA CSHGKFNDAK 
241:	FVFATFNNET GWKNGGIAFI VGLINPAWSF SCLDCATHMA FEVEKPERVI PIAIMGTVAI 
301:	GFVTSFCYVI AMFFSIQDLD AVLSSTTGAP ILDIYNQALG NKSGAIFLGC LILFTSFGCV 
361:	IACHTWQARL CWSFARDNGL PLSRLWSQVN PHTGVPLNAH LMSCAWITLI GLLYLASSTA 
421:	FQSLITGCIA FLLLSYIIPV ICLLAKKRNI AHGPFWLGKF GFFSNIVLLG WTVFSVVFFS 
481:	FPPVLPVTKD NMNYVCVVIV GYTAYSILYW KYKGKKEFHA LEESENEQAE YSNNFDTIED 
541:	SREFSVAASD VELENEHVPW GKK