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2.A.3.4.5
The polyamine (putrescine > spermidine > spermine) exporter, Tpo5p (Ykl174c) [found in the Golgi or post-Golgi secretory vesicles; induction:spermine > spermidine > putrescine] (Igarashi and Kashiwagi 2010).

Accession Number:P36029
Protein Name:Top5p
Length:618
Molecular Weight:69243.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Golgi apparatus membrane1 / Multi-pass membrane protein2
Substrate Putrescine, spermidine, spermine

Cross database links:

Genevestigator: P36029
eggNOG: fuNOG05702
HEGENOM: HBG202405
DIP: DIP-4038N
RefSeq: NP_012747.1   
Entrez Gene ID: 853680   
Pfam: PF00324   
KEGG: sce:YKL174C   

Gene Ontology

GO:0000139 C:Golgi membrane
GO:0016021 C:integral to membrane
GO:0015171 F:amino acid transmembrane transporter activity
GO:0015203 F:polyamine transmembrane transporter activity
GO:0006865 P:amino acid transport
GO:0015846 P:polyamine transport
GO:0055085 P:transmembrane transport

References (6)

[1] “Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI.”  Vandenbol M.et.al.   8091858
[2] “Complete DNA sequence of yeast chromosome XI.”  Dujon B.et.al.   8196765
[3] “A proteomics approach to understanding protein ubiquitination.”  Peng J.et.al.   12872131
[4] “Excretion of putrescine and spermidine by the protein encoded by YKL174c (TPO5) in Saccharomyces cerevisiae.”  Tachihara K.et.al.   15668236
[5] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258
[6] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
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FASTA formatted sequence
1:	MPEYTLLADN IRENIVHFDP NGLFDNLHTI VHEDDSQENE EAEHFNYDQV LDKSLLSRGS 
61:	IVGLGLGLMS PVLGMCTSMA IGLINGGPLT IMLGFLISGV CIWFSSLSLG EIVSKFPMEL 
121:	HVGSAMLAPE KLKLVCSWYT GWLMLIGNWT MSTSITFAGA QLTISLILMT NSNLISEAHL 
181:	IFYTVIVFYL VVTVVGLVNL KFARFIETIN KVCVYWIIYA IIFIDILLLV FHKGKFRSLK 
241:	YALFHFDNNL SGYKSAFLSF IIGFQQSNFT LQGFSMLPAL ADEVKVPEKD IPRGMSNAVL 
301:	LSAFSGVIFL IPIMLILPDN DLLFTNHKVL PIVNIFTKST DSVVLSFFLV LLILGNLLFS 
361:	GIGSITTSSR AVYSFSRDQA IPYYDKWTYV EPDSQSKVPK NSVVLSMIIS YFLGLLALIS 
421:	TAAFNAFIGA AVLCLCSATF IPLVLVLFTR RRAIRSAPVK IRYKFGWFIN IVSIVWLLLS 
481:	MVSVCLPTQV PVTFKTMNYA LMVYVFCILV ITGLYFKWGK YNFRLPLADD IKAPIPSDAE 
541:	ETVFELEDSN VEHTLNSGTT VKESVENNSE EGFIKVHPKS STENPFEENE ENVITDYGDE 
601:	HHTAEQEFDL ADDRRYDI