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2.A.3.7.1
Glutamate:γ-aminobutyrate antiporter of 477 aas and 12 TMSs, GadC.  Expression of gadCB in L. lactis in the presence of chloride is increased when the culture pH decreases to low levels, while glutamate stimulated gadCB expression (Sanders et al. 1998). These genes encode a glutamate-dependent acid resistance mechanism that is optimally active when needed for acid neutralization.

Accession Number:O30417
Protein Name:GadC
Length:503
Molecular Weight:55369.00
Species:Lactococcus lactis (subsp [416870]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Gamma-aminobutyrate, glutamate

Cross database links:

eggNOG: COG0531
HEGENOM: HBG514881
RefSeq: YP_001032491.1   
Entrez Gene ID: 4798793   
Pfam: PF00324   
KEGG: llm:llmg_1178   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015171 F:amino acid transmembrane transporter activity
GO:0015297 F:antiporter activity
GO:0006865 P:amino acid transport
GO:0055085 P:transmembrane transport

References (3)

[1] “A chloride-inducible acid resistance mechanism in Lactococcus lactis and its regulation.”  Sanders J.W.et.al.   9484886
[2] “The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363.”  Wegmann U.et.al.   17307855
[3] “Identification of a sodium chloride-regulated promoter in Lactococcus lactis by single-copy chromosomal fusion with a reporter gene.”  Sanders J.W.et.al.   9604892

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNQKKLSLFG FFALTASMVL TVYEYPTFAT SKLHLVFFLL LGGLLWFLPV ALCAAEMATV 
61:	EGWKNGGIFS WVSQTLGERF GFAAIFFQWF QITVGFVTMI YFILGALSYV LNFQALNTDP 
121:	LIKFIGLLII FWGLTFSQLG GTQRTAKLVK AGFVVGIVIP SVILFGLAAA YFIGGNPIEI 
181:	PINSHAFVPD FSQVSTLVVF VSFILAYMGV EASASHINEL ENPKRNYPLA MILLVILAIS 
241:	LDAIGGFSVA AVIPQKELSL SAGVIQTFQT LILHFNHHLG WLVKVIALMI AFGVMGEVSS 
301:	WVVGPSRGMF AAAQRGLLPK FLRKTNTHEV PVPLVMIQGI IVTLWGAVLT FGGGGNNLSF 
361:	LVAISLTVVI YLVGYLLFFI VYFVLIYKKQ NLKRTYNVPG KIIGKTIIAG IGFLLSIFAL 
421:	FISFVPPASI AKNETHTYQM ILLISFVVTA ILPFIIYELH DKKGHDTIEE PTHFKAGDVN 
481:	PAIYPAARGE HHIIKKEEHI LKH