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2.A.3.7.3
Glutamate:GABA antiporter, GadC (YcaM). GadC, transports GABA/Glu only under acidic conditions, with no detectable activity at pH  values higher than 6.5 (Ma et al., 2012). Ma et al. (2012) determined the crystal structure of GadC at 3.1 Å resolution under basic conditions. GadC, comprising 12 TMSs, exists in a closed state, with its carboxy-terminal domain serving as a plug to block an otherwise inward-open conformation. Structural and biochemical analyses revealed the essential transport residues, identified the transport path and suggested a transport mechanism involving the rigid-body rotation of a helical bundle for GadC and other amino acid antiporters.

Accession Number:C8U8G2
Protein Name:Predicted glutamate:gamma-aminobutyric acid antiporter GadC
Length:511
Molecular Weight:55077.00
Species:Escherichia coli O103:H2 (strain 12009 / EHEC) [585395]
Number of TMSs:12
Substrate glutamate

Cross database links:

eggNOG: COG0531 COG0531
HEGENOM: HOG000058004 HOG000058004
Entrez Gene ID: 8477537   
KEGG: eoh:ECO103_1619    eoh:ECO103_1619   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0015171 F:amino acid transmembrane transporter activity

References (2)

[1] “Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coli.”  Ogura Y.et.al.   19815525
[2] “Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coli.”  Ogura Y.et.al.   19815525
Structure:
4dji     

External Searches:

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  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MATSVQTGKA KQLTLLGFFA ITASMVMAVY EYPTFATSGF SLVFFLLLGG ILWFIPVGLC 
61:	AAEMATVDGW EEGGVFAWVS NTLGPRWGFA AISFGYLQIA IGFIPMLYFV LGALSYILKW 
121:	PALNEDPITK TIAALIILWA LALTQFGGTK YTARIAKVGF FAGILLPAFI LIALAAIYLH 
181:	SGAPVAIEMD SKTFFPDFSK VGTLVVFVAF ILSYMGVEAS ATHVNEMSNP GRDYPLAMLL 
241:	LMVAAICLSS VGGLSIAMVI PGNEINLSAG VMQTFTVLMS HVAPEIEWTV RVISALLLLG 
301:	VLAEIASWIV GPSRGMYVTA QKNLLPAAFA KMNKNGVPVT LVISQLVITS IALIILTNTG 
361:	GGNNMSFLIA LALTVVIYLC AYFMLFIGYI VLVLKHPDLK RTFNIPGGKG VKLVVAIVGL 
421:	LTSIMAFIVS FLPPDNIQGD STDMYVELLV VSFLVVLALP FILYAVHDRK GKANTGVTLE 
481:	PINSQNAPKG HFFLHPRARS PHYIVMNDKK H