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2.A.3.8.1
L-type neutral amino acid transporter, LAT1 (Na+-independent) (prefers amino acids with branched or aromatic side chains: Phe, Ile, Leu, Val, Trp, His; catalyzes obligatory exchange with μM affinities on the outside and mM affinities on the inside [1000x difference]). Both LAT1 and LAT2 (2.A.3.8.6) catalyze uptake of S-nitroso-L-cysteine. These and other LAT family members are specifically inhibited by 2-aminobicyclo[2.2.1]heptane-2-carboxylic acid (Li and Whorton, 2005). Mediates tryptophan:kynurenine exchange (Kaper et al., 2007). Also transports thyroid hormones (Kinne et al., 2011).  The chicken orthologue transports thyrold hormones, especially T2, with low affinity (Nele Bourgeois et al. 2016). Transports certain thyroid hormones and their derivatives (Krause and Hinz 2017).

Accession Number:Q63016
Protein Name:LAT1 aka SLC7A5 aka MPE16 aka TA1
Length:512
Molecular Weight:55903.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cytoplasm1 / Multi-pass membrane protein2
Substrate Amino acids, Thyroid hormones, thyroid hormone derivatives

Cross database links:

Genevestigator: Q63016
eggNOG: maNOG05905
RefSeq: NP_059049.1   
Entrez Gene ID: 50719   
Pfam: PF00324   
KEGG: rno:50719   

Gene Ontology

GO:0016324 C:apical plasma membrane
GO:0005829 C:cytosol
GO:0016021 C:integral to membrane
GO:0042605 F:peptide antigen binding
GO:0030154 P:cell differentiation
GO:0007399 P:nervous system development
GO:0015804 P:neutral amino acid transport
GO:0055085 P:transmembrane transport

References (6)

[1] “Expression cloning and characterization of a transporter for large neutral amino acids activated by the heavy chain of 4F2 antigen (CD98).”  Kanai Y.et.al.   9726963
[2] “TA1, a highly conserved oncofetal complementary DNA from rat hepatoma, encodes an integral membrane protein associated with liver development, carcinogenesis, and cell activation.”  Sang J.et.al.   7532544
[3] “Expression of a system L neutral amino acid transporter at the blood-brain barrier.”  Matsuo H.et.al.   11095508
[4] “Transcriptional regulation of the LAT-1/CD98 light chain.”  Padbury J.F.et.al.   15120633
[5] “Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells.”  Fraga S.et.al.   16027961
[6] “L-type amino acid transporter 1-mediated L-leucine transport at the inner blood-retinal barrier.”  Tomi M.et.al.   15980244

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MAVAGAKRRA VAAPATTAAE EERQAREKML EARRGDGADP EGEGVTLQRN ITLINGVAII 
61:	VGTIIGSGIF VTPTGVLKEA GSPGLSLVVW AVCGVFSIVG ALCYAELGTT ISKSGGDYAY 
121:	MLEVYGSLPA FLKLWIELLI IRPSSQYIVA LVFATYLLKP VFPTCPVPEE AAKLVACLCV 
181:	LLLTAVNCYS VKAATRVQDA FAAAKLLALA LIILLGFIQM GKDIGQGDAS NLHQKLSFEG 
241:	TNLDVGNIVL ALYSGLFAYG GWNYLNFVTE EMINPYRNLP LAIIISLPIV TLVYVLTNLA 
301:	YFTTLSTNQM LTSEAVAVDF GNYHLGVMSW IIPVFVGLSC FGSVNGSLFT SSRLFFVGSR 
361:	EGHLPSILSM IHPQLLTPVP SLVFTCVMTL MYAFSRDIFS IINFFSFFNW LCVALAIIGM 
421:	MWLRFKKPEL ERPIKVNLAL PVFFILACLF LIAVSFWKTP LECGIGFAII LSGLPVYFFG 
481:	VWWKNKPKWI LQVIFSVTVL CQKLMQVVPQ ET