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2.A.3.8.12
The Ser/Thr exchange transporter (SteT) (also transports aromatic amino acids with lower efficiency) (Reig et al., 2007). The substrate-bound state of SteT shows increased conformational flexibility and kinetic stability, enabling transport of substrate across the cell membrane (Bippes et al. 2009). TMS8 sculpts the substrate-binding site and undergoes conformational changes during the transport cycle of SteT (Bartoccioni et al., 2010). Mutations allow substrate binding but not translocation. Other mutations stabilize the protein and result in higher production levels (Rodríguez-Banqueri et al. 2016).

Accession Number:O34739
Protein Name:SteT aka YkbA
Length:438
Molecular Weight:47148.00
Species:Bacillus subtilis [1423]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Serine, Threonine, tyrosine, tryptophan, phenylalanine, Histidine

Cross database links:

HEGENOM: HBG714539
RefSeq: NP_389169.1   
Entrez Gene ID: 936518   
Pfam: PF00324   
BioCyc: SUBTI:BSU12860-MONOMER   
KEGG: bsu:BSU12860   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015171 F:amino acid transmembrane transporter activity
GO:0006865 P:amino acid transport
GO:0055085 P:transmembrane transport

References (2)

[1] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377
[2] “Functional and structural characterization of the first prokaryotic member of the L-amino acid transporter (LAT) family: a model for APC transporters.”  Reig N.et.al.   17344220

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MHTEDNGLKK EIGLLFALTL VIGTIIGSGV FMKPGAVLAY SGDSKMALFA WLLGGILTLA 
61:	GGLTIAEIGT QIPKTGGLYT YLEEVYGEFW GFLCGWVQII IYGPAIIGAL GLYFGSLMAN 
121:	LFGWGSGLSK VIGIIAVLFL CVINIIGTKY GGFVQTLTTI GKLIPIACII VFGLWKGDQH 
181:	IFTAVNESIS DMNFGAAILA TLFAYDGWIL LAALGGEMKN PEKLLPRAMT GGLLIVTAIY 
241:	IFINFALLHI LSANEIVTLG ENATSTAATM LFGSIGGKLI SVGIIVSIFG CLNGKVLSFP 
301:	RVSFAMAERK QLPFAEKLSH VHPSFRTPWI AISFQIALAL IMMLISNPDK LSEISIFMIY 
361:	IFYVMAFFAV FILRKRAKGE KRAYSVPLYP FMPILAIAGS FFVLGSTLIT DTMSCGLSIL 
421:	IGLAGLPVYY GMKKRKAS