TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.3.8.20
Large neutral amino acids transporter small subunit 2 (L-type amino acid transporter 2) (hLAT2) (Solute carrier family 7 member 8). Certain detergents stabilize and allow purification of the 4F2hc-LAT2 complex, allowing the measurement of substrate binding. In addition, an improved 3D map could be obtained (Meury et al. 2014).  Transports many amino acids including thyroid hormones 3',3-T2 and T3 (Hinz et al. 2015; Kinne et al. 2015).

Accession Number:Q9UHI5
Protein Name:Large neutral amino acids transporter small subunit 2
Length:535
Molecular Weight:58382.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cytoplasm1
Substrate T2, T3, Alanine, Asparagine, Cystine, Glutamine, Glycine, Isoleucine, Leucine, Methionine, phenylalanine, Proline, Serine, Threonine, tryptophan, tyrosine, Valine

Cross database links:

Genevestigator: Q9UHI5
eggNOG: COG0531
Entrez Gene ID: 23428   
Drugbank: Drugbank Link   
KEGG: hsa:23428   

Gene Ontology

GO:0016323 C:basolateral plasma membrane
GO:0005737 C:cytoplasm
GO:0005887 C:integral to plasma membrane
GO:0015179 F:L-amino acid transmembrane transporter activity
GO:0015175 F:neutral amino acid transmembrane transporter activity
GO:0015101 F:organic cation transmembrane transporter activity
GO:0042605 F:peptide antigen binding
GO:0019534 F:toxin transporter activity
GO:0007596 P:blood coagulation
GO:0006520 P:cellular amino acid metabolic process
GO:0050900 P:leukocyte migration
GO:0055065 P:metal ion homeostasis
GO:0009636 P:response to toxin

References (12)

[1] “Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids.”  Pineda M.et.al.   10391915
[2] “LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine.”  Rossier G.et.al.   10574970
[3] “SLC7A7, encoding a putative permease-related protein, is mutated in patients with lysinuric protein intolerance.”  Borsani G.et.al.   10080183
[4] “Reabsorption of neutral amino acids mediated by amino acid transporter LAT2 and TAT1 in the basolateral membrane of proximal tubule.”  Park S.Y.et.al.   15918515
[5] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[6] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[7] “Association of 4F2hc with light chains LAT1, LAT2 or y+LAT2 requires different domains.”  Broeer A.et.al.   11311135
[8] “Transport of a neurotoxicant by molecular mimicry: the methylmercury-L-cysteine complex is a substrate for human L-type large neutral amino acid transporter (LAT) 1 and LAT2.”  Simmons-Willis T.A.et.al.   12117417
[9] “CD98 and intracellular adhesion molecule I regulate the activity of amino acid transporter LAT-2 in polarized intestinal epithelia.”  Liu X.et.al.   12716892
[10] “Identification and functional characterization of a Na(+)-independent large neutral amino acid transporter (LAT2) on ARPE-19 cells.”  Gandhi M.D.et.al.   15081149
[11] “Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells.”  Fraga S.et.al.   16027961
[12] “Identification of stereoselective transporters for S-nitroso-L-cysteine: role of LAT1 and LAT2 in biological activity of S-nitrosothiols.”  Li S.et.al.   15769744

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MEEGARHRNN TEKKHPGGGE SDASPEAGSG GGGVALKKEI GLVSACGIIV GNIIGSGIFV 
61:	SPKGVLENAG SVGLALIVWI VTGFITVVGA LCYAELGVTI PKSGGDYSYV KDIFGGLAGF 
121:	LRLWIAVLVI YPTNQAVIAL TFSNYVLQPL FPTCFPPESG LRLLAAICLL LLTWVNCSSV 
181:	RWATRVQDIF TAGKLLALAL IIIMGIVQIC KGEYFWLEPK NAFENFQEPD IGLVALAFLQ 
241:	GSFAYGGWNF LNYVTEELVD PYKNLPRAIF ISIPLVTFVY VFANVAYVTA MSPQELLASN 
301:	AVAVTFGEKL LGVMAWIMPI SVALSTFGGV NGSLFTSSRL FFAGAREGHL PSVLAMIHVK 
361:	RCTPIPALLF TCISTLLMLV TSDMYTLINY VGFINYLFYG VTVAGQIVLR WKKPDIPRPI 
421:	KINLLFPIIY LLFWAFLLVF SLWSEPVVCG IGLAIMLTGV PVYFLGVYWQ HKPKCFSDFI 
481:	ELLTLVSQKM CVVVYPEVER GSGTEEANED MEEQQQPMYQ PTPTKDKDVA GQPQP