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2.A.3.8.25
Large neutral amino acids transporter small subunit 1 (4F2 light chain) (4F2 LC) (4F2LC) (CD98 light chain) (Integral membrane protein E16) (L-type amino acid transporter 1) (hLAT1) (Solute carrier family 7 member 5) (y+ system cationic amino acid transporter).  The heavy chain, CD98hc, modulates integrin signaling, plays a role in cell-to-cell fusion, and is essential for Brucella infection (Keriel et al. 2015).  In addition to L-amino acids, Lat1 in conjunction with 4F2hc, transports S-nitroso-L-cysteine (Li and Whorton 2007).

Accession Number:Q01650
Protein Name:Large neutral amino acids transporter small subunit 1
Length:507
Molecular Weight:55010.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cytoplasm1
Substrate Amino acids, S-nitroso-L-cysteine

Cross database links:

Genevestigator: Q01650
HEGENOM: HOG000098892
Entrez Gene ID: 8140   
KEGG: hsa:8140   

Gene Ontology

GO:0016324 C:apical plasma membrane
GO:0005829 C:cytosol
GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015179 F:L-amino acid transmembrane transporter activity
GO:0015175 F:neutral amino acid transmembrane transporter activity
GO:0042605 F:peptide antigen binding
GO:0007596 P:blood coagulation
GO:0030154 P:cell differentiation
GO:0006520 P:cellular amino acid metabolic process
GO:0006811 P:ion transport
GO:0050900 P:leukocyte migration
GO:0007399 P:nervous system development

References (24)

[1] “Amino-acid transport by heterodimers of 4F2hc/CD98 and members of a permease family.”  Mastroberardino L.et.al.   9751058
[2] “Human LAT1, a subunit of system L amino acid transporter: molecular cloning and transport function.”  Prasad P.D.et.al.   10049700
[3] “Primary structure of the light chain of fusion regulatory protein-1/CD98/4F2 predicts a protein with multiple transmembrane domains that is almost identical to the amino acid transporter E16.”  Tsurudome M.et.al.   10072483
[4] “Human L-type amino acid transporter 1 (LAT1): characterization of function and expression in tumor cell lines.”  Yanagida O.et.al.   11557028
[5] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[6] “A novel transiently expressed, integral membrane protein linked to cell activation. Molecular cloning via the rapid degradation signal AUUUA.”  Gaugitsch H.W.et.al.   1597461
[7] “Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids.”  Pineda M.et.al.   10391915
[8] “LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine.”  Rossier G.et.al.   10574970
[9] “Association of 4F2hc with light chains LAT1, LAT2 or y+LAT2 requires different domains.”  Broeer A.et.al.   11311135
[10] “Role of the System L permease LAT1 in amino acid and iodothyronine transport in placenta.”  Ritchie J.W.A.et.al.   11389679
[11] “Thyroid hormone transport by the heterodimeric human system L amino acid transporter.”  Friesema E.C.H.et.al.   11564694
[12] “Expression and regulation of 4F2hc and hLAT1 in human trophoblasts.”  Okamoto Y.et.al.   11742812
[13] “Transport of a neurotoxicant by molecular mimicry: the methylmercury-L-cysteine complex is a substrate for human L-type large neutral amino acid transporter (LAT) 1 and LAT2.”  Simmons-Willis T.A.et.al.   12117417
[14] “Characterization of the system L amino acid transporter in T24 human bladder carcinoma cells.”  Kim D.K.et.al.   12225859
[15] “Identification and functional characterization of a Na+-independent large neutral amino acid transporter, LAT1, in human and rabbit cornea.”  Jain-Vakkalagadda B.et.al.   12824232
[16] “Large-scale characterization of HeLa cell nuclear phosphoproteins.”  Beausoleil S.A.et.al.   15302935
[17] “Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells.”  Fraga S.et.al.   16027961
[18] “Identification of stereoselective transporters for S-nitroso-L-cysteine: role of LAT1 and LAT2 in biological activity of S-nitrosothiols.”  Li S.et.al.   15769744
[19] “Global phosphoproteome analysis on human HepG2 hepatocytes using reversed-phase diagonal LC.”  Gevaert K.et.al.   16097034
[20] “L-type amino acid transporter 1 as a potential molecular target in human astrocytic tumors.”  Nawashiro H.et.al.   16496379
[21] “Quantitative analysis of global ubiquitination in HeLa cells by mass spectrometry.”  Meierhofer D.et.al.   18781797
[22] “Functional characterization of tyrosine transport in fibroblast cells from healthy controls.”  Vumma R.et.al.   18262359
[23] “Large-scale proteomics analysis of the human kinome.”  Oppermann F.S.et.al.   19369195
[24] “Initial characterization of the human central proteome.”  Burkard T.R.et.al.   21269460

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MAGAGPKRRA LAAPAAEEKE EAREKMLAAK SADGSAPAGE GEGVTLQRNI TLLNGVAIIV 
61:	GTIIGSGIFV TPTGVLKEAG SPGLALVVWA ACGVFSIVGA LCYAELGTTI SKSGGDYAYM 
121:	LEVYGSLPAF LKLWIELLII RPSSQYIVAL VFATYLLKPL FPTCPVPEEA AKLVACLCVL 
181:	LLTAVNCYSV KAATRVQDAF AAAKLLALAL IILLGFVQIG KGDVSNLDPN FSFEGTKLDV 
241:	GNIVLALYSG LFAYGGWNYL NFVTEEMINP YRNLPLAIII SLPIVTLVYV LTNLAYFTTL 
301:	STEQMLSSEA VAVDFGNYHL GVMSWIIPVF VGLSCFGSVN GSLFTSSRLF FVGSREGHLP 
361:	SILSMIHPQL LTPVPSLVFT CVMTLLYAFS KDIFSVINFF SFFNWLCVAL AIIGMIWLRH 
421:	RKPELERPIK VNLALPVFFI LACLFLIAVS FWKTPVECGI GFTIILSGLP VYFFGVWWKN 
481:	KPKWLLQGIF STTVLCQKLM QVVPQET