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2.A.3.8.6
L-type neutral amino acid transporter, LAT2 (Na+-independent with broad specificity for all L-isomers of neutral amino acids; preferred substrate: Phe, His, Trp, Ile, Val, Leu, Gln, Cys, Ser; catalyzes obligatory exchange with μM affinities on the outside and mM affinities on the inside [1000x difference]). Both LAT2 and LAT1 (2.A.3.8.1) catalyze uptake of S-nitro-L-cysteine (Li and Whorton, 2005). Also transports thyroid hormones (Kinne et al., 2011).

Accession Number:Q9WVR6
Protein Name:LAT2 aka SLC7A8
Length:533
Molecular Weight:58190.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cytoplasm1 / Multi-pass membrane protein2
Substrate phenylalanine, Histidine, tryptophan, Isoleucine, Glutamine, Valine, Leucine, Cystine, Serine, Thyroid hormones, S-nitro-L-cysteine

Cross database links:

Genevestigator: Q9WVR6
eggNOG: roNOG06118
RefSeq: NP_445894.1   
Entrez Gene ID: 84551   
Pfam: PF00324   
KEGG: rno:84551   

Gene Ontology

GO:0016323 C:basolateral plasma membrane
GO:0005737 C:cytoplasm
GO:0016021 C:integral to membrane
GO:0042605 F:peptide antigen binding
GO:0015804 P:neutral amino acid transport
GO:0055085 P:transmembrane transport

References (3)

[1] “Identification and functional characterization of a Na+-independent neutral amino acid transporter with broad substrate selectivity.”  Segawa H.et.al.   10391916
[2] “Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells.”  Fraga S.et.al.   16027961
[3] “L-type amino acid transporter 1-mediated L-leucine transport at the inner blood-retinal barrier.”  Tomi M.et.al.   15980244

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MEKGTRQRNN TAKNHPDRGS DTSPEAEASS GGGGVALKKE IGLVSACGII VGNIIGSGIF 
61:	VSPKGVLENA GSVGLALIVW IVTGVITAVG ALCYAELGVT IPKSGGDYSY VKDIFGGLAG 
121:	FLRLWIAVLV IYPTNQAVIA LTFSNYVLQP LFPTCFPPES GLRLLAAICL LLLTWVNCSS 
181:	VRWATRVQDI FTAGKLLALA LIIIMGVVQI CKGEFFWLEP KNAFENFQEP DIGLVALAFL 
241:	QGSFAYGGWN FLNYVTEELV DPYKNLPRAI FISIPLVTFV YVFANIAYVT AMSPQELLAS 
301:	NAVAVTFGEK LLGVMAWIMP ISVALSTFGG VNGSLFTSSR LFFAGAREGH LPSVLAMIHV 
361:	KRCTPIPALL FTCLSTLLML VTSDMYTLIN YVGFINYLFY GVTVAGQIVL RWKKPDIPRP 
421:	IKISLLFPII YLLFWAFLLI FSLWSEPVVC GIGLAIMLTG VPVYFLGVYW QHKPKCFNDF 
481:	IESLTLVSQK MCVVVYPQEG DSGTEETIDD VEEQHKPIFQ PTPVKDPDSE EQP