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2.A.30.1.1
NaCl/KCl symporter; the orthologue in humans when mutated can be responsible for Bartter syndrome, an autosomal recessive disease (Stechman et al., 2007).

Accession Number:P55016
Protein Name:NKC2 aka SLC12A1 aka NKCC2
Length:1095
Molecular Weight:120597.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate NaCl, KCl

Cross database links:

Genevestigator: P55016
eggNOG: roNOG12496
Pfam: PF00324    PF08403   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0008511 F:sodium:potassium:chloride symporter activity
GO:0006821 P:chloride transport
GO:0006813 P:potassium ion transport
GO:0006814 P:sodium ion transport
GO:0055085 P:transmembrane transport

References (1)

[1] “Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney.”  Gamba G.et.al.   8021284

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSVNIPSNSV PSGASRFQVH VINEGHGSGA AMSDSTDPPH YEETSFGDEA QNRLKISFRP 
61:	GNQECYENFL QTGETAKTDT TFHAYDSHTN TYYLQTFGHN TMDAVPKIEY YRNTGSVSGP 
121:	KVNRPSLQEI HEQLAKNVAV APGSADRVAN GDGMPGDEQA ENKEEDVTGV VKFGWVKGVL 
181:	VRCMLNIWGV MLFIRLSWIV GEAGIGLGVI IIGLSVVVTT LTGISMSAIC TNGVVRGGGA 
241:	YYLISRSLGP EFGGSIGLIF RFANAVRVAM YVVGFAETVV DLLKESDSMM VDPTNDIRII 
301:	GSITVVILLG ISVAGMEWEA KAQVILLVIL LIGIANFFIG TVIPSNNEKK SRGFFNYQAS 
361:	IFAENFGPSF TEGEGFFSVF AIFFPAATGI LAGANISGDL EDPQDAIPRG TMLAIFITTV 
421:	AYIGVAICVR ACVVRDATGS MNDTVVSGMN CNGSAACGLG YDFSRCQHEP CQYGLMNNFQ 
481:	VMSMVSGFGP LITAGIFSAT LSSALASLVS APKVFQALCK DNIFKGLQFF AKGYGKNNEP 
541:	LRGYFLTFVI AMAFILIAEL NVIAPIISNF FLASYALINF SCFHASYAKS PGWRPAYGIY 
601:	NMWVSLFGAI LCCAVMFVIN WWAAVITYVI ELFLYIYVTY KKPDVNWGSS TQALSYVSAL 
661:	DNALELTTVE DHVKNFRPQC IVLTGGPMTR PALLDITHAF TKNSGLCICC EVFVGPRKLC 
721:	VKEMNSGMAK KQAWLMKNKI KAFYAAVAAD CFRDGVRSLL QASGLGRMKP NTLVIGYKKN 
781:	WRKAPLSELE NYVGIIHDAF DFEIGVVIVR ISQGFDISPV LQVQDELEKL EQERLALEAA 
841:	IKDNDCEEGK GGIRGLFKKA GKLNITKPAP KKDSNISTIQ SMHVGEFNQK LVEASAQFKK 
901:	KQGKGTIDVW WLFDDGGLTL LIPYILTLRK KWKDCKLRIY VGGKINRIEE EKISMASLLS 
961:	KFRIKFADIH IIGDINIKPN KESWKVFEEM IEPYRLHESH KDLTTAEKLK RESPWKITDA 
1021:	ELEAVKEKSY RQVRLNELLQ EHSRAANLIV LSLPVARKGS ISDLLYMAWL EILTKNLPPV 
1081:	LLVRGNHKNV LTFYS