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2.A.36.1.2
Na+:H+ antiporter 3 (NHE-3 or NHE3). Regulated by Na+/H+ exchange regulatory cofactors (NHERF; O14745; TC #8.A.24.1.1) (Seidler et al., 2009). Cyclic AMP-mediated endocytosis of intestinal epithelial NHE3 requires binding to synaptotagmin 1 (Musch et al., 2010).  Decreased activity is responsible for congenital Na+ diarrhea in the human brush boarder (Janecke et al. 2015). Reduced functional expression of NHE3, and DRA contribute to Cl- and Na+ stool loss in microvillus inclusion disease (MVID) diarrhea (Kravtsov et al. 2016).

Accession Number:P26433
Protein Name:NAH3 aka Nhe-3 aka SLC9A3 aka NHE3
Length:831
Molecular Weight:93105.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:13
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Na+, H+

Cross database links:

Genevestigator: P26433
eggNOG: roNOG08148
RefSeq: NP_036786.1   
Entrez Gene ID: 24784   
Pfam: PF00999   
KEGG: rno:24784   

Gene Ontology

GO:0031526 C:brush border membrane
GO:0016021 C:integral to membrane
GO:0005515 F:protein binding
GO:0015385 F:sodium:hydrogen antiporter activity
GO:0007623 P:circadian rhythm
GO:0006898 P:receptor-mediated endocytosis
GO:0006885 P:regulation of pH
GO:0002028 P:regulation of sodium ion transport
GO:0051384 P:response to glucocorticoid stimulus
GO:0006814 P:sodium ion transport
GO:0055085 P:transmembrane transport

References (3)

[1] “Molecular cloning of putative members of the Na/H exchanger gene family. cDNA cloning, deduced amino acid sequence, and mRNA tissue expression of the rat Na/H exchanger NHE-1 and two structurally related proteins.”  Orlowski J.et.al.   1577762
[2] “Acute inhibition of Na/H exchanger NHE-3 by cAMP. Role of protein kinase a and NHE-3 phosphoserines 552 and 605.”  Zhao H.et.al.   9933588
[3] “Shank2 associates with and regulates Na+/H+ exchanger 3.”  Han W.et.al.   16293618

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MWHPALGPGW KPLLALAVAV TSLRGVRGIE EEPNSGGSFQ IVTFKWHHVQ DPYIIALWIL 
61:	VASLAKIVFH LSHKVTSVVP ESALLIVLGL VLGGIVWAAD HIASFTLTPT LFFFYLLPPI 
121:	VLDAGYFMPN RLFFGNLGTI LLYAVIGTIW NAATTGLSLY GVFLSGLMGE LKIGLLDFLL 
181:	FGSLIAAVDP VAVLAVFEEV HVNEVLFIIV FGESLLNDAV TVVLYNVFES FVTLGGDAVT 
241:	GVDCVKGIVS FFVVSLGGTL VGVIFAFLLS LVTRFTKHVR IIEPGFVFVI SYLSYLTSEM 
301:	LSLSAILAIT FCGICCQKYV KANISEQSAT TVRYTMKMLA SGAETIIFMF LGISAVDPVI 
361:	WTWNTAFVLL TLVFISVYRA IGVVLQTWIL NRYRMVQLET IDQVVMSYGG LRGAVAYALV 
421:	VLLDEKKVKE KNLFVSTTLI VVFFTVIFQG LTIKPLVQWL KVKRSEQREP KLNEKLHGRA 
481:	FDHILSAIED ISGQIGHNYL RDKWSNFDRK FLSKVLMRRS AQKSRDRILN VFHELNLKDA 
541:	ISYVAEGERR GSLAFIRSPS TDNMVNVDFS TPRPSTVEAS VSYFLRENVS AVCLDMQSLE 
601:	QRRRSIRDTE DMVTHHTLQQ YLYKPRQEYK HLYSRHELTP NEDEKQDKEI FHRTMRKRLE 
661:	SFKSAKLGIN QNKKAAKLYK RERAQKRRNS SIPNGKLPME NLAHNFTIKE KDLELSEPEE 
721:	ATNYEEISGG IEFLASVTKD VASDSGAGID NPVFSPDEDL DPSILSRVPP WLSPGETVVP 
781:	SQRARVQIPN SPSNFRRLTP FRLSNKSVDS FLQADGPEEQ LQPASPESTH M