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2.A.36.5.1
Low-affinity Na+ (K+, Li+ or Cs+):H+ antiporter, Nhx1. It is up-regluated in response to high salt stress conditions (Yang et al. 2018).

Accession Number:Q68KI4
Protein Name:Sodium/hydrogen exchanger 1
Length:538
Molecular Weight:59513.00
Species: [3702]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Vacuole membrane1 / Multi-pass membrane protein2
Substrate Na+, K+, Li+, Cs+, H+

Cross database links:

Genevestigator: Q68KI4
HEGENOM: HBG317096
RefSeq: NP_198067.1   
Entrez Gene ID: 832773   
Pfam: PF00999   
KEGG: ath:AT5G27150   

Gene Ontology

GO:0005789 C:endoplasmic reticulum membrane
GO:0000139 C:Golgi membrane
GO:0016021 C:integral to membrane
GO:0000325 C:plant-type vacuole
GO:0005886 C:plasma membrane
GO:0005774 C:vacuolar membrane
GO:0005516 F:calmodulin binding
GO:0015081 F:sodium ion transmembrane transporter activity
GO:0015385 F:sodium:hydrogen antiporter activity
GO:0048366 P:leaf development
GO:0010351 P:lithium ion transport
GO:0006885 P:regulation of pH
GO:0009651 P:response to salt stress
GO:0006814 P:sodium ion transport
GO:0055085 P:transmembrane transport

References (14)

[1] “The Arabidopsis thaliana proton transporters, AtNhx1 and Avp1, can function in cation detoxification in yeast.”  Gaxiola R.A.et.al.   9990049
[2] “Functional conservation between yeast and plant endosomal Na(+)/H(+) antiporters.”  Quintero F.J.et.al.   10767428
[3] “Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.”  Tabata S.et.al.   11130714
[4] “Salt tolerance conferred by overexpression of a vacuolar Na(+)/H(+) antiport in Arabidopsis.”  Apse M.P.et.al.   10455050
[5] “Arabidopsis thaliana and Saccharomyces cerevisiae NHX1 genes encode amiloride sensitive electroneutral Na(+)/H(+) exchangers.”  Darley C.P.et.al.   10998367
[6] “Engineering salt-tolerant Brassica plants: characterization of yield and seed oil quality in transgenic plants with increased vacuolar sodium accumulation.”  Zhang H.-X.et.al.   11606781
[7] “The Arabidopsis Na(+)/H(+) exchanger AtNHX1 catalyzes low affinity Na(+) and K(+) transport in reconstituted liposomes.”  Venema K.et.al.   11707435
[8] “Differential expression and function of Arabidopsis thaliana NHX Na(+)/H(+) antiporters in the salt stress response.”  Yokoi S.et.al.   12047628
[9] “Regulation of expression of the vacuolar Na(+)/H(+) antiporter gene AtNHX1 by salt stress and abscisic acid.”  Shi H.et.al.   12369629
[10] “Vacuolar cation/H(+) exchange, ion homeostasis, and leaf development are altered in a T-DNA insertional mutant of AtNHX1, the Arabidopsis vacuolar Na(+)/H(+) antiporter.”  Apse M.P.et.al.   14535887
[11] “Topological analysis of a plant vacuolar Na(+)/H(+) antiporter reveals a luminal C-terminus that regulates antiporter cation selectivity.”  Yamaguchi T.et.al.   14530406
[12] “Expression of an Arabidopsis vacuolar sodium/proton antiporter gene in cotton improves photosynthetic performance under salt conditions and increases fiber yield in the field.”  He C.et.al.   16179357
[13] “Vacuolar Na(+)/H(+) antiporter cation selectivity is regulated by calmodulin from within the vacuole in a Ca2(+)- and pH-dependent manner.”  Yamaguchi T.et.al.   16249341
[14] “Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks.”  Reiland S.et.al.   19376835

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MLDSLVSKLP SLSTSDHASV VALNLFVALL CACIVLGHLL EENRWMNESI TALLIGLGTG 
61:	VTILLISKGK SSHLLVFSED LFFIYLLPPI IFNAGFQVKK KQFFRNFVTI MLFGAVGTII 
121:	SCTIISLGVT QFFKKLDIGT FDLGDYLAIG AIFAATDSVC TLQVLNQDET PLLYSLVFGE 
181:	GVVNDATSVV VFNAIQSFDL THLNHEAAFH LLGNFLYLFL LSTLLGAATG LISAYVIKKL 
241:	YFGRHSTDRE VALMMLMAYL SYMLAELFDL SGILTVFFCG IVMSHYTWHN VTESSRITTK 
301:	HTFATLSFLA ETFIFLYVGM DALDIDKWRS VSDTPGTSIA VSSILMGLVM VGRAAFVFPL 
361:	SFLSNLAKKN QSEKINFNMQ VVIWWSGLMR GAVSMALAYN KFTRAGHTDV RGNAIMITST 
421:	ITVCLFSTVV FGMLTKPLIS YLLPHQNATT SMLSDDNTPK SIHIPLLDQD SFIEPSGNHN 
481:	VPRPDSIRGF LTRPTRTVHY YWRQFDDSFM RPVFGGRGFV PFVPGSPTER NPPDLSKA