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2.A.36.6.6
Na+/H+ antiporter 1 (MjNhaP1).  NhaP1 is a dimer with 13 TMSs per monomer as revealed by electron crystalography of 2-d crystals (Goswami et al. 2011).  This structure is contrasted with that of the distantly related bacterial NhaA; these two structures are quite different in detail, but similar within the 6 TMS repeat unit. Asp234/235 of helix VIII are involved in ligand-binding, and helix X plays a role in the activation of the transporter (Kedrov et al. 2007).

Accession Number:Q60362
Protein Name:Na(+)/H(+) antiporter 1
Length:426
Molecular Weight:45966.00
Species:Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) [243232]
Number of TMSs:13
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Na+, H+

Cross database links:

eggNOG: COG0025
Entrez Gene ID: 1450896   
Pfam: PF00999   
KEGG: mja:MJ_0057   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015299 F:solute:hydrogen antiporter activity
GO:0006814 P:sodium ion transport

References (3)

[1] “Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.”  Bult C.J.et.al.   8688087
[2] “Identification of a pH regulated Na(+)/H(+) antiporter of Methanococcus jannaschii.”  Hellmer J.et.al.   12220668
[3] “Conserved arginine and aspartate residues are critical for function of MjNhaP1, a Na+/H+ antiporter of M. jannaschii.”  Hellmer J.et.al.   12729893
Structure:
4CZB   4D0A     

External Searches:

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  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MELMMAIGYL GLALVLGSLV AKIAEKLKIP DIPLLLLLGL IIGPFLQIIP SDSAMEIFEY 
61:	AGPIGLIFIL LGGAFTMRIS LLKRVIKTVV RLDTITFLIT LLISGFIFNM VLNLPYTSPV 
121:	GYLFGAITAA TDPATLIPVF SRVRTNPEVA ITLEAESIFN DPLGIVSTSV ILGLFGLFSS 
181:	SNPLIDLITL AGGAIVVGLL LAKIYEKIII HCDFHEYVAP LVLGGAMLLL YVGDDLLPSI 
241:	CGYGFSGYMA VAIMGLYLGD ALFRADDIDY KYIVSFCDDL SLLARVFIFV FLGACIKLSM 
301:	LENYFIPGLL VALGSIFLAR PLGVFLGLIG SKHSFKEKLY FALEGPRGVV PAALAVTVGI 
361:	EILKNADKIP ASITKYITPT DIAGTIIIGT FMTILLSVIL EASWAGMLAL KLLGEYKPKY 
421:	KEESHH