TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.39.2.2
The vitamin B6:H+ symporter, Tpn1 (pyridoxine, pyridoxal and pyridoxamine are substrates) (Stolz and Vielreicher, 2003)

Accession Number:P53099
Protein Name:Tpn1 of Saccharomyces cerevisiae
Length:579
Molecular Weight:64546.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Vitamin B6, H+, Pyridoxine, Pyridoxal, Pyridoxamine

Cross database links:

Genevestigator: P53099
eggNOG: fuNOG05349
HEGENOM: HBG395679
RefSeq: NP_011329.1   
Entrez Gene ID: 852689   
Pfam: PF02133   
KEGG: sce:YGL186C   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015205 F:nucleobase transmembrane transporter activity
GO:0051183 F:vitamin transporter activity
GO:0015931 P:nucleobase, nucleoside, nucleotide and nucl...
GO:0051180 P:vitamin transport

References (4)

[1] “Tpn1p, the plasma membrane vitamin B6 transporter of Saccharomyces cerevisiae.”  Stolz J.et.al.   12649274
[2] “Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae.”  Coglievina M.et.al.   9046087
[3] “The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.”  Tettelin H.et.al.   9169869
[4] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MNRDNMDTTK RKEDHTKHTT DVIEFYEEGT AASSLNIATE KANSSPSILR RIINRAAWLS 
61:	KKVDAMGVES TGIQRISPYE RGTSKKQFLH VAGLWLSATG GLSSMSSFLL GPLLFGLSFR 
121:	ESVASSLISV TIGCLIAAYC SIMGPQSGCR QMVTARYLFG WWFVKLVALA SIIGVMGWSV 
181:	VNSVVGGEML AAISNDKVPL WVGIVIVTVC SFLVAIFGIK QVIKVETYLS VPVLTAFLLL 
241:	YISSSDKYSF VNAYVSKGNL DSSTRKGNWM SFFSLCYSIT ATWGSITADY YILFPEDTPY 
301:	IQIFCLTFFG TFLPTCFVGI LGLLLASVAM SYKPWSVEYD SHGMGGLLWA GFQRWNGFGK 
361:	FCVVVLVFSL VSNNIINTYS AAFSIQLSSV FCAKIPRWFW SIVCTIICLV CALIGRNHFS 
421:	TILGNFLPMI GYWISMYFIL LFEENLVFRR FFLHLYTKEF PTVTGEINGP ELVGSSKEVE 
481:	KDAVTNIHLL KRKHKVTKHR YNWDKWEDYE VLTHGYAATF AFIVGVAGVV VGMAQAYWIG 
541:	PIAAKFGEYG GDVAMWLSMA FSGVVYPPCR YLELRKFGR