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2.A.39.3.4
Allantoin permease, PucI, with 12 TMSs and both N- and C-termini on the inside. PucI transports allantoin with a Km of 24 mμM, but recognizes some additional hydantoin compounds, including hydantoin itself, and to a lesser extent a range of nucleobases and nucleosides (Ma et al. 2016).

Accession Number:P94575
Protein Name:PucI aka YwoE
Length:490
Molecular Weight:53982.00
Species:Bacillus subtilis [1423]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Allantoin

Cross database links:

HEGENOM: HBG616138
RefSeq: NP_391528.1   
Entrez Gene ID: 936935   
Pfam: PF02133   
KEGG: bsu:BSU36470   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015205 F:nucleobase transmembrane transporter activity
GO:0015931 P:nucleobase, nucleoside, nucleotide and nucl...
GO:0055085 P:transmembrane transport

References (3)

[1] “The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).”  Presecan E.et.al.   9353933
[2] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377
[3] “Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.”  Schultz A.C.et.al.   11344136

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKLKESQQQS NRLSNEDLVP LGQEKRTWKA MNFASIWMGC IHNIPTYATV GGLIAIGLSP 
61:	WQVLAIIITA SLILFGALAL NGHAGTKYGL PFPVIIRASY GIYGANIPAL LRAFTAIMWL 
121:	GIQTFAGSTA LNILLLNMWP GWGEIGGEWN ILGIHLSGLL SFVFFWAIHL LVLHHGMESI 
181:	KRFEVWAGPL VYLVFGGMVW WAVDIAGGLG PIYSQPGKFH TFSETFWPFA AGVTGIIGIW 
241:	ATLILNIPDF TRFAETQKEQ IKGQFYGLPG TFALFAFASI TVTSGSQVAF GEPIWDVVDI 
301:	LARFDNPYVI VLSVITLCIA TISVNVAANI VSPAYDIANA LPKYINFKRG SFITALLALF 
361:	TVPWKLMESA TSVYAFLGLI GGMLGPVAGV MMADYFIIRK RELSVDDLYS ETGRYVYWKG 
421:	YNYRAFAATM LGALISLIGM YVPVLKSLYD ISWFVGVLIS FLFYIVLMRV HPPASLAIET 
481:	VEHAQVRQAE