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2.A.4.2.1
Mitochondrial Co2+/Zn2+ uptake (into mitochondria) permease.  A single mutation (N45I) increases the specificity for Fe2+ and decreases it for Co2+ (Lin et al., 2008).

Accession Number:P32798
Protein Name:Cotl aka YOR316C aka O6131
Length:439
Molecular Weight:48155.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:5
Location1 / Topology2 / Orientation3: Mitochondrion membrane1 / Multi-pass membrane protein2
Substrate Co2+, Zn2+, Fe2+

Cross database links:

Genevestigator: P32798
eggNOG: fuNOG05089
HEGENOM: HBG317426
DIP: DIP-7554N
RefSeq: NP_014961.1   
Entrez Gene ID: 854494   
Pfam: PF01545   
KEGG: sce:YOR316C   

Gene Ontology

GO:0000324 C:fungal-type vacuole
GO:0016021 C:integral to membrane
GO:0031966 C:mitochondrial membrane
GO:0015087 F:cobalt ion transmembrane transporter activity
GO:0005515 F:protein binding
GO:0005385 F:zinc ion transmembrane transporter activity
GO:0006882 P:cellular zinc ion homeostasis
GO:0006824 P:cobalt ion transport
GO:0055085 P:transmembrane transport
GO:0006829 P:zinc ion transport

References (7)

[1] “COT1, a gene involved in cobalt accumulation in Saccharomyces cerevisiae.”  Conklin D.S.et.al.   1508175
[2] “Sequencing of a 35.71 kb DNA segment on the right arm of yeast chromosome XV reveals regions of similarity to chromosomes I and XIII.”  Pearson B.M.et.al.   8896266
[3] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.”  Dujon B.et.al.   9169874
[4] “Global analysis of protein expression in yeast.”  Ghaemmaghami S.et.al.   14562106
[5] “A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.”  Hitchcock A.L.et.al.   14557538
[6] “Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.”  Chi A.et.al.   17287358
[7] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKLGSKQVKI ISLLLLDTVF FGIEITTGYL SHSLALIADS FHMLNDIISL VVALWAVNVA 
61:	KNRNPDSTYT YGWKRAEILG ALINAVFLIA LCVSILIEAL QRIIAPPVIE NPKFVLYVGV 
121:	AGLISNTVGL FLFHDNDQEH GHGHGHSHGG IFADHEMHMP SSHTHTHAHV DGIENTTPMD 
181:	STDNISEIMP NAIVDSFMNE NTRLLTPENA SKTPSYSTSS HTIASGGNYT EHNKRKRSLN 
241:	MHGVFLHVLG DALGNIGVML SAFFIWKTDY SWKYYTDPLV SLIITGIIFS SALPLSCKAS 
301:	KILLQATPST LSGDQVEGDL LKIPGIIAIH DFHIWNLTES IFIASLHIQL DISPEQFTDL 
361:	AKIVRSKLHR YGIHSATLQP EFITREVTST ERAGDSQGDH LQNDPLSLRP KTYGTGISGS 
421:	TCLIDDAANC NTADCLEDH