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2.A.40.3.3
Uric acid (urate) permease YgfU. YgfU exhibits low affinity (0.5 mM) but high capacity for urate and very poor activity for xanthine.  Essential residues were identified.  Coversion of Thr-100 to ala resulted in efficient xanthine transport (Papakostas and Frillingos 2012).  

Accession Number:Q46821
Protein Name:Putative purine permease ygfU
Length:482
Molecular Weight:51758.00
Species:Escherichia coli (strain K12) [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Urate, Xanthine

Cross database links:

Genevestigator: Q46821
EchoBASE: EB2882
EcoGene: EG13071
eggNOG: COG2233
HEGENOM: HOG000038199
DIP: DIP-12176N
Entrez Gene ID: 949017   
Pfam: PF00860   
KEGG: eco:b2888   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity
GO:0055085 P:transmembrane transport

References (3)

[1] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[2] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[3] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSAIDSQLPS SSGQDRPTDE VDRILSPGKL IILGLQHVLV MYAGAVAVPL MIGDRLGLSK 
61:	EAIAMLISSD LFCCGIVTLL QCIGIGRFMG IRLPVIMSVT FAAVTPMIAI GMNPDIGLLG 
121:	IFGATIAAGF ITTLLAPLIG RLMPLFPPLV TGVVITSIGL SIIQVGIDWA AGGKGNPQYG 
181:	NPVYLGISFA VLIFILLITR YAKGFMSNVA VLLGIVFGFL LSWMMNEVNL SGLHDASWFA 
241:	IVTPMSFGMP IFDPVSILTM TAVLIIVFIE SMGMFLALGE IVGRKLSSHD IIRGLRVDGV 
301:	GTMIGGTFNS FPHTSFSQNV GLVSVTRVHS RWVCISSGII LILFGMVPKM AVLVASIPQF 
361:	VLGGAGLVMF GMVLATGIRI LSRCNYTTNR YNLYIVAISL GVGMTPTLSH DFFSKLPAVL 
421:	QPLLHSGIML ATLSAVVLNV FFNGYQHHAD LVKESVSDKD LKVRTVRMWL LMRKLKKNEH 
481:	GE