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2.A.40.4.3
The YgfO (XanQ) purine (xanthine) transporter. Residues involved in substrate binding have been identified (Georgopoulou et al., 2010). TMS3 functions in substrate recognition (Karena and Frillingos, 2011).  Many more essential residues have more recently been identified (Karena et al. 2015).

Accession Number:P67444
Protein Name:Xanthine permease XanQ
Length:466
Molecular Weight:49108.00
Species:Escherichia coli (strain K12) [83333]
Number of TMSs:13
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Xanthine

Cross database links:

Genevestigator: P67444 P67444
EchoBASE: EB2877 EB2877
EcoGene: EG13065 EG13065
eggNOG: COG2233 COG2233
HEGENOM: HBG470187 HBG470187
Entrez Gene ID: 949075   
Pfam: PF00860   
KEGG: ecj:JW2850    eco:b2882   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0042907 F:xanthine transmembrane transporter activity

References (6)

[1] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[2] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[3] “Cloning and functional characterization of two bacterial members of the NAT/NCS2 family in Escherichia coli.”  Karatza P.et.al.   16096267
[4] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
[5] “Cysteine-scanning analysis of putative helix XII in the YgfO xanthine permease: ILE-432 and ASN-430 are important.”  Papakostas K.et.al.   18359771
[6] “Role of intramembrane polar residues in the YgfO xanthine permease: HIS-31 and ASN-93 are crucial for affinity and specificity, and ASP-304 and GLU-272 are irreplaceable.”  Karena E.et.al.   19581302

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSDINHAGSD LIFELEDRPP FHQALVGAIT HLLAIFVPMV TPALIVGAAL QLSAETTAYL 
61:	VSMAMIASGI GTWLQVNRYG IVGSGLLSIQ SVNFSFVTVM IALGSSMKSD GFHEELIMSS 
121:	LLGVSFVGAF LVVGSSFILP YLRRVITPTV SGIVVLMIGL SLIKVGIIDF GGGFAAKSSG 
181:	TFGNYEHLGV GLLVLIVVIG FNCCRSPLLR MGGIAIGLCV GYIASLCLGM VDFSSMRNLP 
241:	LITIPHPFKY GFSFSFHQFL VVGTIYLLSV LEAVGDITAT AMVSRRPIQG EEYQSRLKGG 
301:	VLADGLVSVI ASAVGSLPLT TFAQNNGVIQ MTGVASRYVG RTIAVMLVIL GLFPMIGGFF 
361:	TTIPSAVLGG AMTLMFSMIA IAGIRIIITN GLKRRETLIV ATSLGLGLGV SYDPEIFKIL 
421:	PASIYVLVEN PICAGGLTAI LLNIILPGGY RQENVLPGIT SAEEMD