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2.A.41.1.1
Pyrimidine nucleoside:H+ symporter, NupC (Craig et al. 1994; Patching et al. 2005). Wild-type NupC had an apparent affinity for uridine of 22.2 +/- 3.7 muM and an apparent binding affinity of 1.8-2.6 mM, and various mutants with alterred properties were isolated and characterized (Sun et al. 2017).

Accession Number:P0AFF2
Protein Name:Nucleoside-transport system protein NupC aka CRU aka B2393
Length:400
Molecular Weight:43476.00
Species:Escherichia coli [83333]
Number of TMSs:9
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Nucleosides, H+

Cross database links:

Genevestigator: P0AFF2
EchoBASE: EB1914
EcoGene: EG11971
eggNOG: COG1972
HEGENOM: HBG713614
RefSeq: AP_002992.1    NP_416894.1   
Entrez Gene ID: 946895   
Pfam: PF07662    PF01773   
BioCyc: EcoCyc:NUPC-MONOMER    ECOL168927:B2393-MONOMER   
KEGG: ecj:JW2389    eco:b2393   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005415 F:nucleoside:sodium symporter activity
GO:0006810 P:transport

References (5)

[1] “Cloning of the nupC gene of Escherichia coli encoding a nucleoside transport system, and identification of an adjacent insertion element, IS 186.”  Craig J.E.et.al.   8022285
[2] “Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.”  Yamamoto Y.et.al.   9205837
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MDRVLHFVLA LAVVAILALL VSSDRKKIRI RYVIQLLVIE VLLAWFFLNS DVGLGFVKGF 
61:	SEMFEKLLGF ANEGTNFVFG SMNDQGLAFF FLKVLCPIVF ISALIGILQH IRVLPVIIRA 
121:	IGFLLSKVNG MGKLESFNAV SSLILGQSEN FIAYKDILGK ISRNRMYTMA ATAMSTVSMS 
181:	IVGAYMTMLE PKYVVAALVL NMFSTFIVLS LINPYRVDAS EENIQMSNLH EGQSFFEMLG 
241:	EYILAGFKVA IIVAAMLIGF IALIAALNAL FATVTGWFGY SISFQGILGY IFYPIAWVMG 
301:	VPSSEALQVG SIMATKLVSN EFVAMMDLQK IASTLSPRAE GIISVFLVSF ANFSSIGIIA 
361:	GAVKGLNEEQ GNVVSRFGLK LVYGSTLVSV LSASIAALVL