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2.A.41.2.3
Pyrimidine-preferring nucleoside:Na+ symporter, CNT1 (Na+/nucleoside = 2)(transports uridine, gemcitabine and 5'-deoxy-5'-fluorouridine) (Larráyoz et al., 2004)

Accession Number:O00337
Protein Name:CNT1 aka SLC28A1
Length:649
Molecular Weight:71584.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:15
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Uridine, Gemcitabine, 5'-deoxy-5'-fluorouridine, Na+

Cross database links:

COG1972
Genevestigator: O00337 O00337
RefSeq: NP_004204.3    NP_964014.1   
Entrez Gene ID: 9154   
Pfam: PF07670    PF07662    PF01773   
OMIM: 606207  gene
KEGG: hsa:9154    hsa:9154   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0005624 C:membrane fraction
GO:0001882 F:nucleoside binding
GO:0005415 F:nucleoside:sodium symporter activity
GO:0006139 P:nucleobase, nucleoside, nucleotide and nucl...
GO:0015858 P:nucleoside transport
GO:0005337 F:nucleoside transmembrane transporter activity
GO:0006139 P:nucleobase-containing compound metabolic process

References (10)

[1] “Molecular cloning and functional expression of cDNAs encoding a human Na+-nucleoside cotransporter (hCNT1).”  Ritzel M.W.L.et.al.   9124315
[2] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[3] “Analysis of the DNA sequence and duplication history of human chromosome 15.”  Zody M.C.et.al.   16572171
[4] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[5] “Identification of amino acid residues responsible for the pyrimidine and purine nucleoside specificities of human concentrative Na(+) nucleoside cotransporters hCNT1 and hCNT2.”  Loewen S.K.et.al.   10455109
[6] “Molecular cloning and functional expression of cDNAs encoding a human Na+-nucleoside cotransporter (hCNT1).”  Ritzel M.W.L.et.al.   9124315
[7] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[8] “Analysis of the DNA sequence and duplication history of human chromosome 15.”  Zody M.C.et.al.   16572171
[9] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[10] “Identification of amino acid residues responsible for the pyrimidine and purine nucleoside specificities of human concentrative Na(+) nucleoside cotransporters hCNT1 and hCNT2.”  Loewen S.K.et.al.   10455109

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MENDPSRRRE SISLTPVAKG LENMGADFLE SLEEGQLPRS DLSPAEIRSS WSEAAPKPFS 
61:	RWRNLQPALR ARSFCREHMQ LFRWIGTGLL CTGLSAFLLV ACLLDFQRAL ALFVLTCVVL 
121:	TFLGHRLLKR LLGPKLRRFL KPQGHPRLLL WFKRGLALAA FLGLVLWLSL DTSQRPEQLV 
181:	SFAGICVFVA LLFACSKHHC AVSWRAVSWG LGLQFVLGLL VIRTEPGFIA FEWLGEQIRI 
241:	FLSYTKAGSS FVFGEALVKD VFAFQVLPII VFFSCVISVL YHVGLMQWVI LKIAWLMQVT 
301:	MGTTATETLS VAGNIFVSQT EAPLLIRPYL ADMTLSEVHV VMTGGYATIA GSLLGAYISF 
361:	GIDATSLIAA SVMAAPCALA LSKLVYPEVE ESKFRREEGV KLTYGDAQNL IEAASTGAAI 
421:	SVKVVANIAA NLIAFLAVLD FINAALSWLG DMVDIQGLSF QLICSYILRP VAFLMGVAWE 
481:	DCPVVAELLG IKLFLNEFVA YQDLSKYKQR RLAGAEEWVG DRKQWISVRA EVLTTFALCG 
541:	FANFSSIGIM LGGLTSMVPQ RKSDFSQIVL RALFTGACVS LVNACMAGIL YMPRGAEVDC 
601:	MSLLNTTLSS SSFEIYQCCR EAFQSVNPEF SPEALDNCCR FYNHTICAQ