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2.A.47.2.2
Vacuolar low affinity phosphate transporter, Pho91 (Estrella et al., 2008). Also transports selenite (Lazard et al., 2010). Pyrophosphate stimulates the phosphate-sodium symporter of Trypanosoma brucei (TC# 2.A.47.2.4) acidocalcisomes and Saccharomyces cerevisiae vacuoles (this protein) (Potapenko et al. 2019).

Accession Number:P27514
Protein Name:Pho91
Length:894
Molecular Weight:99490.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate phosphate, Selenite

Cross database links:

Genevestigator: P27514
eggNOG: fuNOG04553
HEGENOM: HBG329859
DIP: DIP-4264N
RefSeq: NP_014410.1   
Entrez Gene ID: 855747   
Pfam: PF00939    PF03105   
KEGG: sce:YNR013C   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005774 C:vacuolar membrane
GO:0015114 F:phosphate transmembrane transporter activity
GO:0006817 P:phosphate transport
GO:0006797 P:polyphosphate metabolic process
GO:0006814 P:sodium ion transport
GO:0055085 P:transmembrane transport

References (8)

[1] “Twelve open reading frames revealed in the 23.6 kb segment flanking the centromere on the Saccharomyces cerevisiae chromosome XIV right arm.”  Verhasselt P.et.al.   7900425
[2] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.”  Philippsen P.et.al.   9169873
[3] “The URK1 gene of Saccharomyces cerevisiae encoding uridine kinase.”  Kern L.et.al.   2169608
[4] “Global analysis of protein expression in yeast.”  Ghaemmaghami S.et.al.   14562106
[5] “A proteomics approach to understanding protein ubiquitination.”  Peng J.et.al.   12872131
[6] “Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.”  Li X.et.al.   17330950
[7] “Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases.”  Smolka M.B.et.al.   17563356
[8] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKFSHSLQFN SVPEWSTKYL AYSQLKKLIY SLQKDKLYSN NKHHVVEPHD ANDENLPLLA 
61:	DASPDDQFYI SKFVAALNQE LKKIDKFYIS QETGLIANYN ELKDDVMELE NTNKATQLFN 
121:	QQQQHQLQSV ARNRKSKSQQ RQRRFSSVSS TDSNPSLTDM SIDSAPVIHT QVSNTTNNGN 
181:	SMQNLASASV SLSNSNPVYL SPFTQHRLSL KKRLISIYTQ LSELKDFIEL NQTGFSKICK 
241:	KFDKSLNTNL KQNYLNYIKF HSHVFNPATI NRIQHHITET ILTYASLNKG TRRPSNTFNL 
301:	DADRINNDEN SSGNEEDEDG NRQEVLDFQD AERELSSHLR DHVVWERNTV WKDMMNLERK 
361:	YQSAKTDNKK FSKLSSSQLR PNANITESMA MSSGGAGIIA PSTDSLTFRE LMHLPPKQWL 
421:	QFIMGQTSLL KFLLITSCFI ALLTFNLTPF TQDSLQKNCF AILIYASLLW ATETIPLFVT 
481:	SLMIPLLIVV FPVIKDPITS QPMSPRDSSQ FILSTMWSSV IMLLLGGFTL AAALSKYNIA 
541:	KVLSTHILAS AGTNPHFILL TNMFVALFVS MWVSNVAAPV LCYSIVQPLL RTLPRNCSYA 
601:	KALILGIALA SNIGGMSSPI ASPQNIFSIG IMDPSPSWAE WFMIALPVCF ICVMAIWVLL 
661:	IITFPPEPNV KILQLHPSRD PFTLKQWFVT LVCIITIVLW CLSNQISGIF GEMGIISIIP 
721:	IVVFFGTGLL TSDDFNNFMW TIVVLAMGGT TLGKAVSSSG LLSTMAQLIK AQVEHEPIFI 
781:	IVLIFGLVIL VMATFVSHTV AAMIIVPLMS EIGSNLPSGD HSRLLIVIAA LLCSSAMGLP 
841:	TSGFPNVTAI SMIDEVGDRY LTVGTFITRG VPASLLSYAA IVTVGYGILK VMGF