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2.A.47.3.2
Citrate:succinate antiporter (Pos et al. 1998).  Binds and presumably regulates the heterodimeric citrate lyase, CitE/CitF which converts citrate to succinate and acetate (Quentmeier et al. 1987).  These proteins form a metabolon which together catalyze citrate fermentation under anaerobic conditions.

Accession Number:P0AE74
Protein Name:Citrate carrier CitT aka B0612
Length:487
Molecular Weight:53093.00
Species:Escherichia coli [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Citrate, Succinate

Cross database links:

Genevestigator: P0AE74
EchoBASE: EB3308
EcoGene: EG13538
eggNOG: COG0471
HEGENOM: HBG321139
RefSeq: AP_001259.1    NP_415145.1   
Entrez Gene ID: 949070   
Pfam: PF00939   
BioCyc: EcoCyc:B0612-MONOMER    ECOL168927:B0612-MONOMER   
KEGG: ecj:JW0604    eco:b0612   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015297 F:antiporter activity
GO:0006814 P:sodium ion transport
GO:0055085 P:transmembrane transport

References (4)

[1] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “The Escherichia coli citrate carrier CitT: a member of a novel eubacterial transporter family related to the 2-oxoglutarate/malate translocator from spinach chloroplasts.”  Pos K.M.et.al.   9696764

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSLAKDNIWK LLAPLVVMGV MFLIPVPDGM PPQAWHYFAV FVAMIVGMIL EPIPATAISF 
61:	IAVTICVIGS NYLLFDAKEL ADPAFNAQKQ ALKWGLAGFS STTVWLVFGA FIFALGYEVS 
121:	GLGRRIALFL VKFMGKRTLT LGYAIVIIDI LLAPFTPSNT ARTGGTVFPV IKNLPPLFKS 
181:	FPNDPSARRI GGYLMWMMVI STSLSSSMFV TGAAPNVLGL EFVSKIAGIQ ISWLQWFLCF 
241:	LPVGVILLII APWLSYVLYK PEITHSEEVA TWAGDELKTM GALTRREWTL IGLVLLSLGL 
301:	WVFGSEVINA TAVGLLAVSL MLALHVVPWK DITRYNSAWN TLVNLATLVV MANGLTRSGF 
361:	IDWFAGTMST HLEGFSPNAT VIVLVLVFYF AHYLFASLSA HTATMLPVIL AVGKGIPGVP 
421:	MEQLCILLVL SIGIMGCLTP YATGPGVIIY GCGYVKSKDY WRLGAIFGVI YISMLLLVGW 
481:	PILAMWN