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2.A.49.2.11
The voltage-gated torpedo chloride channel, CLC-0, of 810 aas. This channel is thought to ensure the high conductance of the non-innervated membrane of the electrocyte necessary for efficient current generation caused by sodium influx through the acetylcholine receptor at the innervated membrane.  ClC-0 is a homodimer with separate pores in each subunit. Each protopore can be opened and closed independently from the other pore by a ""fast gate"". A common, slow gate acts on both pores simultaneously. The opening of the fast gate is controlled by a critical glutamate (E166), whose protonation state determines the fast gate's pH dependence (Zifarelli and Pusch 2010). Extracellular protons likely arrive directly at E166, but protonation of E166 from the inside may be mediated by the dissociation of an intrapore water molecule. The OH- anion resulting from dissociation of water is stabilized in one of the anion binding sites of the channel, competing with intracellular Cl-. Proton translocation drives irreversible gating transitions associated with the slow gate (Zifarelli and Pusch 2010). The structure of the cytoplasmic domain has been solved (Meyer and Dutzler 2006). It contains a folded core of two tightly interacting cystathionine beta-synthetase (CBS) subdomains which are connected by a 96 residue mobile linker. The domains form dimers, thereby extending the 2-fold symmetry of the transmembrane pore.  

Accession Number:P35522
Protein Name:Chloride channel protein
Length:810
Molecular Weight:89447.00
Species:Torpedo californica (Pacific electric ray) [7787]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Cl-

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FASTA formatted sequence
1:	MSHEKNEASG NPEAQSWKAQ EAMLGVKTEV SRWRAVKNCL YRHLVKVLGE DWIFLLLLGA 
61:	LMALVSWAMD FIGSRGLRFY KYLFAMVEGN LGLQYLVWVC YPLILILFSS LFCQIVSPQA 
121:	VGSGIPELKT IIRGAVLHEY LTLRTFVAKT VGLTVALSAG FPLGKEGPFV HIASICATLL 
181:	NQLLCFISGR REEPYYLRAD ILTVGCALGI SCCFGTPLAG VLFSIEVTCS HFGVRSYWRG 
241:	FLGGAFSAFI FRVLSVWVKD TVTLTALFKT NFRGDIPFDL QELPAFAIIG IASGFFGALF 
301:	VYLNRQIIVF MRKKNFVTKI LKKQRLIYPA VVTFVLATLR FPPGVGQFFG AGLMPRETIN 
361:	SLFDNYTWTK TIDPRGLGNS AQWFIPHLNI FIVMALYFVM HFWMAALAVT MPVPCGAFVP 
421:	VFNLGAVLGR FVGELMALLF PDGLVSNGNL YHILPGEYAV IGAAAMTGAV THAVSTAVIC 
481:	FELTGQISHV LPMMVAVILA NMVAQGLQPS LYDSIIQIKK LPYLPELSWS SANKYNIQVG 
541:	DIMVRDVTSI ASTSTYGDLL HVLRQTKLKF FPFVDTPETN TLLGSIERTE VEGLLQRRIS 
601:	AYRRQPATAA EAEEEGRNGE RGASFTGDVP GEAETSFAYI DQEEAEGQQQ REGLEAVKVQ 
661:	TEDPRPPSPV PAEEPTQTSG IYQKKHKGTG QVASRFEEML TLEEIYQWEQ REKNVVVNFE 
721:	TCRIDQSPFQ LVEGTSLQKT HTLFSLLGLD RAYVTSMGKL VGVVALAEIQ AAIEGSYQKG 
781:	FRLPPPLASF RDAKNARNSG RTATSNSSGK