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2.A.49.2.4
The kidney medulla voltage-gated dimeric chloride channel, ClC-K1 or CLCNKA.  Regulated by dehydration; functions in the regulation of cell volume, membrane potential stabilization, signal transduction and transepithelial transport.  Possibly required for urinary concentration in the kidney (Uchida et al., 1993).  The human orthologue when mutated can give rise to salt loss associated with Bartter''s syndrome. In the inner ear, it plays a role in hearing. Activators and inhibitors that may have theraputic benefits are known (Gradogna and Pusch 2010). Regulated by the accessory subunit, Barttin (Wojciechowski et al. 2015).

Accession Number:Q06393
Protein Name:Chloride channel protein ClC-Ka aka ClC-K1
Length:687
Molecular Weight:75569.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Cl-

Cross database links:

Genevestigator: Q06393
eggNOG: roNOG07385
RefSeq: NP_445779.1   
Entrez Gene ID: 79425   
Pfam: PF00571    PF00654   
KEGG: rno:79425   

Gene Ontology

GO:0016324 C:apical plasma membrane
GO:0016323 C:basolateral plasma membrane
GO:0034707 C:chloride channel complex
GO:0005783 C:endoplasmic reticulum
GO:0005887 C:integral to plasma membrane
GO:0005247 F:voltage-gated chloride channel activity
GO:0007589 P:body fluid secretion
GO:0006821 P:chloride transport
GO:0001822 P:kidney development
GO:0051592 P:response to calcium ion
GO:0009268 P:response to pH
GO:0009414 P:response to water deprivation
GO:0055085 P:transmembrane transport

References (5)

[1] “Molecular cloning of a chloride channel that is regulated by dehydration and expressed predominantly in kidney medulla.”  Uchida S.et.al.   7680033
[2] “Molecular cloning of a chloride channel that is regulated by dehydration and expressed predominantly in kidney medulla.”  Uchida S.et.al.   8034678
[3] “Two highly homologous members of the ClC chloride channel family in both rat and human kidney.”  Kieferle S.et.al.   8041726
[4] “Barttin increases surface expression and changes current properties of ClC-K channels.”  Waldegger S.et.al.   12111250
[5] “Parallel down-regulation of chloride channel CLC-K1 and barttin mRNA in the thin ascending limb of the rat nephron by furosemide.”  Wolf K.et.al.   12759757

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MEELVGLREG SSGKPVTLQE LWGPCPRIRR GVRRGLEWLK ERLFRVGEDW HFLVALGVLM 
61:	ALISYAMNFA IGRVVRAHKW LYREVGDGHL LRYLSWTVYP VALLSFSSGF SQSISPFSGG 
121:	SGLPELKTML SGVVLEDYLD IKNFGAKVVG LSCTLATGST IFLGKVGPFV HLSVMISAYL 
181:	GRVRAKTIGE TENKAKEIEM LSAAAAVGVA TVFAAPFSGV LFSIEVMSSH FSVWNYWRGF 
241:	FAATCGAFMF RLLGVFNSEQ ETITSIYKTR FRVDVPFDLP EIFFFVALGF ICGVLSCAYL 
301:	FCQRTFLRFI KTNRYTSRLL ATSKPSYAAL VALVLASITY PPGVGRFMAS RLSMAEHLHS 
361:	LFDNNSWALM TRNSSPPWPA EPDPQNLWLE WCHPRFTIFG TLAFFLVMKF WMLILATTIP 
421:	MPAGYFMPIF IIGAAIGRLL GEALSVAFPE GIVAGREVNP IMPGGYALAG AAAFSGAVTH 
481:	TISTALLAFE LTGQIVHALP VLMAVLAANA ISQNCQPSFY DGTIMAKKLP YLPWIRGRQI 
541:	GSYPVTVEHF MNCNLTTLAK DTPLEEVVKV VTSTEVSQYP LVETRESQTL VGIVERTHLV 
601:	QALQTQPASW APGQERFLQD ILAGGCPTQP VTLQLSPETS LYQAHSLFER LTLQSLFVTS 
661:	RGKAVGSVSW AELKKAISTL INPPAPK