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2.A.49.2.5
The kidney thick ascending limb of Henle's loop and collecting duct chloride channel/carrier, ClC-K2 (Br- > I- > Cl- >> cyclamate) (Adachi et al., 1994).  Regulated by the accessory subunit, Barttin (Wojciechowski et al. 2015).

Accession Number:P51802
Protein Name:Chloride channel protein ClC-Kb aka ClC-K2
Length:687
Molecular Weight:75218.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Cl-, Br-, I-, Cyclamate

Cross database links:

Genevestigator: P51802
eggNOG: roNOG07385
RefSeq: NP_775126.1   
Entrez Gene ID: 79430   
Pfam: PF00571    PF00654   
KEGG: rno:79430   

Gene Ontology

GO:0034707 C:chloride channel complex
GO:0000139 C:Golgi membrane
GO:0005886 C:plasma membrane
GO:0005247 F:voltage-gated chloride channel activity
GO:0030321 P:transepithelial chloride transport
GO:0055085 P:transmembrane transport

References (4)

[1] “Two highly homologous members of the ClC chloride channel family in both rat and human kidney.”  Kieferle S.et.al.   8041726
[2] “Two isoforms of a chloride channel predominantly expressed in thick ascending limb of Henle's loop and collecting ducts of rat kidney.”  Adachi S.et.al.   8021279
[3] “Barttin increases surface expression and changes current properties of ClC-K channels.”  Waldegger S.et.al.   12111250
[4] “Molecular mechanisms of Bartter syndrome caused by mutations in the BSND gene.”  Hayama A.et.al.   12761627

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
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FASTA formatted sequence
1:	MEEIVGLREG SPRKPVPLQE LWRPCPRIRR NIQGSLEWLK ERLFRVGEDW YFLVALGVLM 
61:	ALISYAMNFA IGRVVRAHKW LYREIGDGHL LRYLSWTVYP VALLSFSSGF SQSITPSSGG 
121:	SGIPEVKTIL TGVILEDYLD IKNFGAKVVG LSCTLATGST IFLGKLGPFV HLSVMIAAYL 
181:	GRVRTKTVGE PENKTKEMEL LAAGAAVGVA TVFAAPISGV LFSIEVMSSH FSVWDYWRGF 
241:	FAATCGAFMF HLLAVFNSEQ ETITSIYKTS FPVDIPFDLP EIFFFVALGA ICGILSCGYN 
301:	YCQRTSLFFL KSNGFTSKLL ATSKPLYSAL AAVVLASITY PPGVGRFMAS RLSMSEYLET 
361:	LFDNNSWALM TKNSSPPWSA EPDPQNLWLE WCHPQMTVFG TLVFFLVMKF WMLILATTIP 
421:	IPAGYFLPIF VYGAAIGRLF GEVLSLAFPE GIVAGGKVSP IMPGAYALAG AAAFSGAVTH 
481:	TLSTALLAFE VSGQIVHALP VLMAVLAANA ICQSYQPSFY DGTIIVKKLP YLPWIRGRKI 
541:	GSHLVTVGHF MNCTLTTLAK DMPLEEVIKV VISTDVTQYP LVETTESQVL VGIVKRTHLV 
601:	QSLHTDSASW APGQQPCLQD ILANGCPTQP VTLQLSTETS LHETHNLFEL LNLQLLFVTS 
661:	RGRAVGSVSW VELKKAISTL TNPPAPK