TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.49.2.6
The ClC-2 or CLCN2 chloride channel is expressed in many tissues (Cl- ≥ Br- > I-) (Thiemann et al., 1992). Regulated in the retina by Cereblon (Q96SW2) (Hohberger and Enz, 2009). A toxin, GaTx2, is a potent and specific inhibitor of ClC-2, binding with a Ki of 20pM (Thompson et al., 2009). ClC-2 channels constitute part of the background conductance and assist chloride extrusion (Rinke et al., 2010). ClC-2 channels regulate neuronal excitability,vision and fertility, but not intracellular chloride levels (Ratté and Prescott, 2011; Sánchez-Rodríguez et al. 2012).  Sequential interactions of chloride and proton ions with the fast gate steer voltage-dependent gating (Sánchez-Rodríguez et al. 2012).  The detailed mechanism of proton gating of chloride currents has been presented (Niemeyer et al. 2009).

Accession Number:P35525
Protein Name:Chloride channel protein 2 aka CIC-2 aka Clcn2
Length:907
Molecular Weight:99328.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Cl-, Br-, I-

Cross database links:

Genevestigator: P35525
RefSeq: NP_058833.1   
Entrez Gene ID: 29232   
Pfam: PF00571    PF00654   
KEGG: rno:29232   

Gene Ontology

GO:0015629 C:actin cytoskeleton
GO:0034707 C:chloride channel complex
GO:0030425 C:dendrite
GO:0005626 C:insoluble fraction
GO:0043204 C:perikaryon
GO:0005247 F:voltage-gated chloride channel activity
GO:0006821 P:chloride transport
GO:0030324 P:lung development
GO:0055085 P:transmembrane transport

References (1)

[1] “A chloride channel widely expressed in epithelial and non-epithelial cells.”  Thiemann A.et.al.   1311421

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MAAATAAAAT VAGEGMEPRA LQYEQTLMYG RYTQELGAFA KEEAARIRLG GPEPWKGSPS 
61:	ARATPELLEY GQSRCARCRI CSVRCHKFLV SRVGEDWIFL VLLGLLMALV SWAMDYAIAV 
121:	CLQAQQWMSR GLNTNILLQY LAWVTYPVVL ITFSAGFTQI LAPQAVGSGI PEMKTILRGV 
181:	VLKEYLTLKT FVAKVIGLTC ALGSGMPLGK EGPFVHIASM CAALLSKFLS LFGGIYENES 
241:	RNTEMLAAAC AVGVGCCFAA PIGGVLFSIE VTSTFFAVRN YWRGFFAATF SAFIFRVLAV 
301:	WNRDEETITA LFKTRFRLDF PFDLQELPAF AVIGIASGFG GALFVYLNRK IVQVMRKQKT 
361:	INRFLMKKRL LFPALVTLLI STLTFPPGFG QFMAGQLSQK ETLVTLFDNR TWVRQGLVED 
421:	LGAPSTSQAW SPPRANVFLT LVIFILMKFW MSALATTIPV PCGAFMPVFV IGAAFGRLVG 
481:	ESMAAWFPDG IHTDSSTYRI VPGGYAVVGA AALAGAVTHT VSTAVIVFEL TGQIAHILPV 
541:	MIAVILANAV AQSLQPSLYD SIIRIKKLPY LPELGWGRHQ QYRVRVEDIM VRDVPHVALS 
601:	CTFRDLRLAL HRTKGRMLAL VESPESMILL GSIERSQVVA LLGAQLSPAR RRQHMQKLRK 
661:	AQMSPPSDQE SPPSSETSIR FQVNTEDSGF PGAHGQTHKP LKPALKRGPS NATSLQEGTT 
721:	GNMESAGIAL RSLFCGSPPL ESTTSELEKS ESCDKRKLKR VRISLASDSD LEGKMSPEEI 
781:	LEWEEQQLDE PVNFSDCKID PAPFQLVERT SLHKTHTIFS LLGVDHAYVT SIGRLIGIVT 
841:	LKELRKAIEG SVTAQGVKVR PPLASFRDSA TSSSDTETTE VHALWGPRSR HGLPREGTPS 
901:	DSDDKCQ