TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.5.5.1
Broad specificity heavy metal divalent cation uptake transporter, ZupT (Fe2+, Co2+, Mn2+, Cd2+ and Zn2+ are transported) (Grass et al., 2005). Point mutations change the specificity and kinetics of metal uptake (Taudte and Grass, 2010). Important for virulence in Salmonella (Karlinsey et al., 2010).

Accession Number:P0A8H3
Protein Name:Zinc transporter ZupT
Length:257
Molecular Weight:26485.00
Species:Escherichia coli [83333]
Number of TMSs:8
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Fe2+, Co2+, Mn2+, Cd2+, Zn2+

Cross database links:

Genevestigator: P0A8H3
EchoBASE: EB1155
EcoGene: EG11167
eggNOG: COG0428
HEGENOM: HBG620552
RefSeq: AP_003590.1    NP_417512.1   
Entrez Gene ID: 947035   
Pfam: PF02535   
BioCyc: EcoCyc:EG11167-MONOMER    ECOL168927:B3040-MONOMER   
KEGG: ecj:JW3008    eco:b3040   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0046873 F:metal ion transmembrane transporter activity
GO:0006824 P:cobalt ion transport
GO:0015677 P:copper ion import
GO:0015684 P:ferrous iron transport
GO:0006831 P:low-affinity zinc ion transport
GO:0006828 P:manganese ion transport

References (4)

[1] “Nucleotide sequence of a region duplicated in Escherichia coli toc mutants.”  Yang T.-P.et.al.   1314093
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “ZupT is a Zn(II) uptake system in Escherichia coli.”  Grass G.et.al.   11790762

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MSVPLILTIL AGAATFIGAF LGVLGQKPSN RLLAFSLGFA AGIMLLISLM EMLPAALAAE 
61:	GMSPVLGYGM FIFGLLGYFG LDRMLPHAHP QDLMQKSVQP LPKSIKRTAI LLTLGISLHN 
121:	FPEGIATFVT ASSNLELGFG IALAVALHNI PEGLAVAGPV YAATGSKRTA ILWAGISGLA 
181:	EILGGVLAWL ILGSMISPVV MAAIMAAVAG IMVALSVDEL MPLAKEIDPN NNPSYGVLCG 
241:	MSVMGFSLVL LQTAGIG