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2.A.52.1.1
High affinity Ni2+-specific transporter, HoxN (Hebbeln and Eitinger 2004).

Accession Number:P23516
Protein Name:HoxN
Length:351
Molecular Weight:38771.00
Species:Alcaligenes eutrophus (Ralstonia eutropha) [381666]
Number of TMSs:8
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Ni2+

Cross database links:

HEGENOM: HBG322888
RefSeq: NP_942665.1   
Entrez Gene ID: 2656444   
Pfam: PF03824   
KEGG: reh:PHG023   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0046872 F:metal ion binding
GO:0015099 F:nickel ion transmembrane transporter activity
GO:0015675 P:nickel ion transport
GO:0055085 P:transmembrane transport

References (4)

[1] “Cloning, nucleotide sequence, and heterologous expression of a high-affinity nickel transport gene from Alcaligenes eutrophus.”  Eitinger T.et.al.   1847142
[2] “The Alcaligenes eutrophus protein HoxN mediates nickel transport in Escherichia coli.”  Wolfram L.et.al.   7896709
[3] “Complete nucleotide sequence of pHG1: a Ralstonia eutropha H16 megaplasmid encoding key enzymes of H(2)-based lithoautotrophy and anaerobiosis.”  Schwartz E.et.al.   12948488
[4] “A topological model for the high-affinity nickel transporter of Alcaligenes eutrophus.”  Eitinger T.et.al.   7934894

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MFQLLAGVRM NSTGRPRAKI ILLYALLIAF NIGAWLCALA AFRDHPVLLG TALLAYGLGL 
61:	RHAVDADHLA AIDNVTRKLM QDGRRPITAG LWFSLGHSSV VVLASVLIAV MATTLQERLD 
121:	AFHEVGSVIG TLASALFLFA IAAINLVILR SAYRAFRRVR RGGIYVEEDF DLLFGNRGFL 
181:	ARIFRPLFRF ITRSWHMYPL GMLFALGFDT ATEVALLGIS TMEASRGVPI WSILVFPALF 
241:	TAGMALIDTI DSILMCGAYA WAYAKPVRKL YYNMTITFVS AIVALIVGGI ETLGLLADKF 
301:	MLKGVFWNAV GALNENFCQL GFVIIGIFTV CWVVSIVVYR LRRYDDSEVR A